Overview

  • Product name

    Anti-AVEN antibody [EPR4720]
    See all AVEN primary antibodies
  • Description

    Rabbit monoclonal [EPR4720] to AVEN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human AVEN aa 100-200. The exact sequence is proprietary.

  • Positive control

    • Ramos, HeLa, MCF-7 and Raji cell lysates; Human kidney tissue; HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling AVEN with ab133285 at 1/50 dilution.
    • All lanes : Anti-AVEN antibody [EPR4720] (ab133285) at 1/1000 dilution

      Lane 1 : Ramos cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : Raji cell lysate

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa

    • Immunofluorescent analysis of HeLa cells labelling AVEN with ab133285 at 1/100 dilution.

    References

    This product has been referenced in:

    • O'Doherty AM  et al. Expression of granulosa cell microRNAs, AVEN and ATRX are associated with human blastocyst development. Mol Reprod Dev N/A:N/A (2018). Read more (PubMed: 29693772) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Chicken Tissue sections (Embryonic chick brainstem)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Tween-20 in TBS
    Specification
    Embryonic chick brainstem
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Forrest Weghorst

    Verified customer

    Submitted Dec 07 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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