Recombinant Anti-AVEN antibody [EPR4720] (ab133285)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4720] to AVEN
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-AVEN antibody [EPR4720]
See all AVEN primary antibodies -
Description
Rabbit monoclonal [EPR4720] to AVEN -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human, Mouse and Rat testis tissue; ICC/IF: Ramos cells; Flow Cyt: Ramos cells. WB: Ramos, HeLa, RAW 264.7 and PC-12 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4720 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/40.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/50- 1/100. |
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ICC/IF |
1/50 - 1/250.
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Notes |
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Flow Cyt
1/40. |
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/50- 1/100. |
ICC/IF
1/50 - 1/250. |
Target
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Function
Protects against apoptosis mediated by Apaf-1. -
Tissue specificity
Highly expressed in testis, ovary, thymus, prostate, spleen, small intestine, colon, heart, skeletal muscle, liver, kidney and pancreas. -
Cellular localization
Endomembrane system. Associated with intracellular membranes. - Information by UniProt
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Database links
- Entrez Gene: 57099 Human
- Entrez Gene: 74268 Mouse
- Entrez Gene: 311299 Rat
- Omim: 605265 Human
- SwissProt: Q9NQS1 Human
- SwissProt: Q9D9K3 Mouse
- Unigene: 555966 Human
- Unigene: 315125 Mouse
see all -
Alternative names
- 1700013A01Rik antibody
- 1700056A21Rik antibody
- Apoptosis caspase activation inhibitor antibody
see all
Images
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All lanes : Anti-AVEN antibody [EPR4720] (ab133285) at 1/1000 dilution (Purified)
Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling AVEN with purified ab133285 at 1/4000 dilution (0.11 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunocytochemistry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling AVEN with purified ab133285 at 1/50 dilution (8.7 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labelling AVEN with purified ab133285 at 1/40 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling AVEN with purified ab133285 at 1/4000 dilution (0.11 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling AVEN with purified ab133285 at 1/4000 dilution (0.11 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab133285 has been referenced in 1 publication.
- O'Doherty AM et al. Expression of granulosa cell microRNAs, AVEN and ATRX are associated with human blastocyst development. Mol Reprod Dev N/A:N/A (2018). PubMed: 29693772