Overview

  • Product name
    Anti-Avian Influenza A Hemagglutinin antibody
    See all Avian Influenza A Hemagglutinin primary antibodies
  • Description
    Rabbit polyclonal to Avian Influenza A Hemagglutinin
  • Host species
    Rabbit
  • Specificity
    Rabbit anti-Hemagglutinin (IN) protein polyclonal antibody was raised against a synthetic peptide corresponding to 14 amino acids in the middle of the Hemagglutinin protein (Genbank accession no. AAT76166). Efforts were made to use relatively conserved regions of the viral sequence as the antigen. The antibody only recognizes the full-length H5, not the cleaved subunit.
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with avian Influenza A virus.
  • Immunogen

    A synthetic peptide corresponding to 14 amino acids from an internal sequence of the Hemagglutinin protein conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab21292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 µg/ml. to detect 10 ng of free peptide.

Target

  • Relevance
    Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • HA1 antibody
    • HA2 antibody
    • Hemagglutinin antibody
    • Hemagglutinin ha1 chain antibody
    • Hemagglutinin ha2 chain antibody
    • Hemagglutinin precursor antibody
    see all

References

This product has been referenced in:
  • Wang R  et al. Interdigitated array microelectrode based impedance immunosensor for detection of avian influenza virus H5N1. Talanta 79:159-64 (2009). Read more (PubMed: 19559858) »
See 1 Publication for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for contacting us. For clarification, can you please tell me if you are interested in the Avian Influenza A Hemagglutinin peptide, ab39771, or the antibody that was raised against it, ab21292, or both?

The amino acid sequence of the peptide is considered to be proprietary information.

In general, modifications to the formulations of proteins and antibodies require a minimum order of 10 units, if the modified formulation is available at all. (Availability varies depending on the product).

We do have "cleanup kits" for sale that will remove azide and BSA from antibodies, but they are not applicable to peptides.

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102784).

Click here (or use the following: https://www.abcam.com/index.html?datasheet=102776).

I look forward to your reply.

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Answer

Thank you for your enquiry. Those residues are not part of the immunogen for this antibody. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for your message and your valuable feedback which has now been passed on to me in the Technical Support department. I am very sorry to hear you have had problems obtaining satisfactory results using these antibodies. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to our questionnaires which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. Although ab21297 and ab21292 are used in ELISA, ab2285 can be used in several applications. I have therefore provided links to each type of application so you can choose the correct one to fill out for each antibody. https://www.abcam.com/index.html?section=elisa&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=western&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ihc&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ip&pageconfig=technical&mode=questionaire Thank you for your time. I look forward to receiving the completed questionnaires.

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Question
Answer

Thank you for your enquiry. The peptide was conjugated to KLH prior to immunization. Please let me know if you have any more questions.

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Answer

Thank you for your enquiry. Unfortunately, I cannot disclose the immunogen sequence used to generate this antibody. The antibody is against a peptide selected from the middle-third of the H5 precursor with the following accession numbers: Genbank accession number AAT76166 Swiss-Prot accession number Q692M2 Please let me know if you have any more questions.

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Answer

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your email. The immunogen sequence used is proprietary and cannot be released. The antibody was raised against a synthetic peptide corresponding to 14 amino acids in the middle of the Hemagglutinin protein (Genbank accession no. AAT76166). A highly conserved region from the H5 protein was selected so it will cross-react with multiple strains. Please contact us again if you have any additional questions. Happy holidays!

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Answer

Thank you for your enquiry and your interest in our products. We ran a sequence alignment between AAT76166 (what we used for the H5N1 antigen) and CAD38288 (H7N1) and got the following results: 5 identical residues, 2 highly conserved and 7 residues have no homology. This is not enough for the antibody to cross-react. There are multiple H7N1 sequences out there, so we ran a BLAST against the immunogen as well and found that it is specific to H5N1. We hope this helps.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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