Overview

  • Product name
    Anti-Avian Influenza A Hemagglutinin antibody
    See all Avian Influenza A Hemagglutinin primary antibodies
  • Description
    Rabbit polyclonal to Avian Influenza A Hemagglutinin
  • Host species
    Rabbit
  • Specificity
    Rabbit anti-Hemagglutinin (NT) protein polyclonal antibody was raised against a synthetic peptide corresponding to 15 amino acids at the amino terminus of the Hemagglutinin protein (Genbank accession no. AAT76166). Efforts were made to use relatively conserved regions of the viral sequence as the antigen. The antibody only recognizes the cleaved subunit not the full-length H5.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with Avian Influenza A (H5N1) Hemagglutinin Not yet tested in other species
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the N terminus of the Hemagglutinin protein. (Peptide available as ab39772).

Properties

Applications

Our Abpromise guarantee covers the use of ab21297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.Can be blocked with Chicken Avian Influenza A Hemagglutinin peptide (ab39772).
ELISA Use a concentration of 1 µg/ml.

Target

  • Relevance
    Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • HA1 antibody
    • HA2 antibody
    • Hemagglutinin antibody
    • Hemagglutinin ha1 chain antibody
    • Hemagglutinin ha2 chain antibody
    • Hemagglutinin precursor antibody
    see all

Images

  • H5N1 HA Antibody (ab21297 at 1 µg/ml) specifically recognizes Avian Influenza A (H5N1), but not seasonal influenza A (H1N1), recombinant Hemagglutinin protein in ELISA.
  • Western blot analysis of 5 ng (A) or 25 ng (B) of recombinant Hemagglutinin (HA1) using H5N1 Hemagglutinin antibody (ab21297 at 1 µg/ml.)

References

This product has been referenced in:
  • Lin SC  et al. Glycan masking of hemagglutinin for adenovirus vector and recombinant protein immunizations elicits broadly neutralizing antibodies against H5N1 avian influenza viruses. PLoS One 9:e92822 (2014). WB . Read more (PubMed: 24671139) »
  • Lin SC  et al. Broader Neutralizing Antibodies against H5N1 Viruses Using Prime-Boost Immunization of Hyperglycosylated Hemagglutinin DNA and Virus-Like Particles. PLoS One 7:e39075 (2012). WB . Read more (PubMed: 22720032) »
See all 5 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

I can confirm that ab21292 only recognizes the full-length H5 and ab21297 will recognize a cleaved subunit. Unfortunately, I cannot disclose the exact peptide sequence. I hope this information will help you,

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Answer

Thank you for your message and your valuable feedback which has now been passed on to me in the Technical Support department. I am very sorry to hear you have had problems obtaining satisfactory results using these antibodies. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to our questionnaires which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. Although ab21297 and ab21292 are used in ELISA, ab2285 can be used in several applications. I have therefore provided links to each type of application so you can choose the correct one to fill out for each antibody. https://www.abcam.com/index.html?section=elisa&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=western&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ihc&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ip&pageconfig=technical&mode=questionaire Thank you for your time. I look forward to receiving the completed questionnaires.

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Question
Answer

Thank you for your enquiry. I have been in touch with the source of this antibody and I can tell you that unfortunately the immunogen sequence is proprietary. However, I can tell you that in their opinion the antibody should not cross-react to other Hemagglutinins. Please let me know if you have any more questions.

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Question
Answer

Thank you for your enquiry. Unfortunately, the originator of the antibody will not disclose this information. The most specific information I can give you is what is listed on our datasheet, that the immunogen was selected from between residues 100-150 of Genbank accession number AAT76166. I am sorry I could not be more helpful. Please do not hesitate to contact us again if you need anything further.

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Answer

Thank you for your enquiry. I have performed an alignment of the immunogen sequence with accession numbers AAW80717, AAT39066, and AAA43083. I can tell you that the immunogen was selected from a conserved region of the sequence and shows the following sequence similarity: AAW80717: *************** (100% homology) AAT39066: *************** (100% homology) AAA43083: *************** (100% homology) Please let me know if you have any more questions.

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Answer

Thank you for your enquiry. Unfortunately I cannot provide you with details of the accession number that was used for the design of the immunogen for this antibody. However, I can perform an alignment of your H5 amino acid sequence with the immunogen sequence and provide you with details of the degree of sequence similarity. If you would like me to do this please e-mail me the accession number of your H5 sequence. I look forward to hearing from you.

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Answer

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for your enquiry. I can not provide you with the exact sequence used, but it is 14 amino acids within residues 100-150 for ab21297 and within residues 300-350 for catalog number ab21292. Please let me know if you have any more questions.

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Question
Answer

Thank you for your enquiry. Unfortunately I do not have details of any cross-reactivity by this antiserum against non-H5N1 hemagglutinin proteins. I have blasted the peptide sequence of the 15 N-terminal residues: MERIVIALAIISIVK derived from the EXPASY database: http://us.expasy.org/uniprot/Q7TEA9 and there are no results suggesting that this sera was raised using a specific immunogen. I am sorry I cannot be of more help.

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Answer

Thank you for your enquiry. Ab21297 was generated using a synthetic peptide corresponding to 15 amino acids at the N-terminus of the Hemagglutinin protein. The exact sequence is proprietary. The antibody's specifity was tested by peptide ELISA in which the antibody was able detect 10 ng of free peptide at 1 µg/ml. The antibody reacts with Avian Influenza A (H5N1) Hemagglutinin. If you have any additional questions, please contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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