Question (18492) | Anti-Avian Influenza A Hemagglutinin antibody (ab21297)

Go to datasheet (ab21297)

Question

In your email below (26/08/06) you asked me for feedback about your product. I have given my comments and would like to find out your suggestions about how your antibody could be used, because it clearly doesn't work with our standard protocol. Our antigen is a recombinant A/VietNam/1194/04 and we tried to use the antibody on a monolayer of cells that were either infected or uninfected (see attached data file). Thank you very much for the opportunity to give you feedback regarding your antibodies. At the time I ordered two anti-Influenza A Heamagglutinin(H5N1)polyclonal antibodies (ab21297 and 21292)and both don't work at all in our assay. (data attached) Not only get I no/low signal with your product - it also seems that I get an immense background as well. Meanwhile another member of my group has ordered an antibody (anti-Influenza A Nucleoprotein; ab2285) from your company that also doesn't work (gives the same kind of results as in my experiments). We have received anti- N protein antibodies from several other companies that do work well in ELISA format and I have found that your products are unfit for the advertised purpose.

Answer

Thank you for your message and your valuable feedback which has now been passed on to me in the Technical Support department. I am very sorry to hear you have had problems obtaining satisfactory results using these antibodies. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 90 days of purchase), and if it appears that the antibody is at fault, a credit note/refund will be offered. If you would like some technical support I enclose a link to our questionnaires which will help you put your protocol information together very easily so we can look into the details of the experiment and provide some help. Although ab21297 and ab21292 are used in ELISA, ab2285 can be used in several applications. I have therefore provided links to each type of application so you can choose the correct one to fill out for each antibody. https://www.abcam.com/index.html?section=elisa&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=western&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ihc&pageconfig=technical&mode=questionaire https://www.abcam.com/index.html?section=ip&pageconfig=technical&mode=questionaire Thank you for your time. I look forward to receiving the completed questionnaires.

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