Synthetic peptide corresponding to 12 amino acids near the amino terminus of the Avian influenza A Hemagglutinin 1 protein. Efforts were made to use relatively conserved regions of the viral sequence as the antigen.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. The antibody will detect 10 ng of free peptide at 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability.
Hemagglutinin ha1 chain antibody
Hemagglutinin ha2 chain antibody
Hemagglutinin precursor antibody
Western blot - Anti-Avian Influenza A Hemagglutinin antibody (ab62587)
All lanes : Anti-Avian Influenza A Hemagglutinin antibody (ab62587) at 1 µg/ml
Lane 1 : 5 ng of Recombinant HA1 Lane 2 : 25 ng of Recombinant HA1