Overview

  • Product name
    Anti-Avian Influenza A Neuraminidase antibody
    See all Avian Influenza A Neuraminidase primary antibodies
  • Description
    Rabbit polyclonal to Avian Influenza A Neuraminidase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with Avian Influenza A virus.
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids at the carboxy terminus of the Neuraminidase protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab21304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 21151571

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration. It will detect 10 ng of free peptide at 1 µg/ml.

Target

  • Relevance
    Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
  • Cellular localization
    Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).
  • Alternative names
    • NA antibody
    • Neuraminidase antibody

Images

  • Expression of the H1 or N1 proteins by recombinant vaccinia viruses was detected by Western blotting. Vero cells in case of the VV-L constructs, or the avian cell line DF-1 [15] in case of MVA, were infected at a multiplicity of infection of 0.1 for 48 hours. MVA-H1-Ca or rVVL-H1-Ca infected cells were harvested by scraping or by adding trypsin. MVA-N1-Ca or rVVL-N1-Ca infected cells were harvested by scraping. Sonicated cell lysates were loaded onto 12% polyacrylamide gels and afterwards blotted on nitrocellulose membrane. To detect the H1 protein, a sheep antiserum against the A/California/7/2009 hemagglutinin (NIBSC 09/152) was used. Donkey-anti-sheep alkaline phosphatase-conjugated IgG was used as a secondary antibody. To detect the N1 protein ab21304 was utilized. Goat-anti-rabbit alkaline phosphatase-conjugated IgG was used as a secondary antibody. A whole virus vaccine H1N1 A/California/7/2009 [16] served as positive control.
    Neuraminidase expression in chicken cells.

References

This product has been referenced in:
  • Fan RL  et al. Generation of Live Attenuated Influenza Virus by Using Codon Usage Bias. J Virol 89:10762-73 (2015). WB . Read more (PubMed: 26269186) »
  • Tripathi S  et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis 4:e562 (2013). WB . Read more (PubMed: 23538443) »
See all 5 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Vielen Dank für Ihre Anfrage.

Wir haben unsere Datenblatter nun auf den neuesten Stand gebracht: Sie können nun die SDB direkt als PDF Datei von unserer Netzseite vom jeweiligen Datenblatt herunter laden.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

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Answer

Thank you for your enquiry and your interest.

Tested applications are listed on the on-line product datasheets. Since IHC is not listed on neither of the documents (ab21304 and ab110599), this means that these two productsare not tested and characterized in this specific application. However, this does not necessary imply that the products are unsuitable but we do not have any supporting data yet.

Since both antibodies work in ELISA - an application which recognize the epitope(s) in the natured confirmation - it is very likely that they detect the targets in IHC as well.

If you need any further assistance in the future, please do not hesitate to contact me.

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Answer

Thank you for kindly providing the sequence. This has been checked, and has the following percentage alignment with the following two antibodies: ab21304 Avian Influenza A Neuraminidase antibody: 80% ab21305 Avian Influenza A Neuraminidase antibody: 87% We recommend alignment shoudl be over 85% to predict that an antibody will detect. Therefore, there is likely to be some crossreactivity with ab21304, but ab21305 may be a better choice based on the alignment scores. Please check the online datasheet to ensure this has been tested in the application the customer would like to use. Click here (or use the following: https://www.abcam.com/index.html?datasheet=21305). I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Goat Recombinant protein (LMH cells)
Loading amount
10 µg
Specification
LMH cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 02 2010

Answer

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. A BLAST search on the sequence reveals that Ab21304 should cross-react with neuraminidase from the following strains: H1N1,H4N1, H6N1, H7N1, H9N1 and H11N1. If you have any further questions then please get back in contact with me.

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Answer

Thank you for getting back to me. Once again, unfortunately I cannot comment on the peptide sequence that this antiserum was raised against as it remains proprietary. However, given the sequence alignment that you have performed it seems that there is significant sequence similarity, in particular within the C terminal end (residues 400-469 show only two differences, both highly conserved). Given this I would expect this antiserum to work against the 1918 Spanish flu. Furthermore given that this is a polyclonal it is even more likely to accommodate any subtle change in epitope should the epitope lie within one of the two residue differences. I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Answer

Thank you for your enquiry. Unfortunately the immunogen for this antibody remains proprietary. The peptide sequence used to generate ab21304 is not from the very C-terminus, but from the C-terminal region. It has 13/15 (*******:*****.*) identical residues, 1 highly conserved and 1 conserved when compared to the corresponding region in human InFluenza Virus NA. I was unable to find the sequence for 1918 Spanish Flu NA in NCBI's database. However, I did some searches for it through Google and found a putative sequence through literature (AF117241). If this is the sequence for the 1918 Spanish Flu NA protein there are only two identical residues, and 6 conserved residues: * : :*::: : Please note that AF117241 has very low similarity with the CAC95053 sequence: http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20051004-18542419&poll=yes I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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