Key features and details
- Rabbit polyclonal to Avian Influenza A Neuraminidase
- Suitable for: WB, ELISA
- Isotype: IgG
Product nameAnti-Avian Influenza A Neuraminidase antibody
See all Avian Influenza A Neuraminidase primary antibodies
DescriptionRabbit polyclonal to Avian Influenza A Neuraminidase
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with Avian Influenza A virus.
Synthetic peptide corresponding to 15 amino acids at the carboxy terminus of the Neuraminidase protein.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab21304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml.|
|ELISA||Use at an assay dependent concentration. It will detect 10 ng of free peptide at 1 µg/ml.|
RelevanceCatalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
Cellular localizationCell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).
- NA antibody
- Neuraminidase antibody
Expression of the H1 or N1 proteins by recombinant vaccinia viruses was detected by Western blotting. Vero cells in case of the VV-L constructs, or the avian cell line DF-1  in case of MVA, were infected at a multiplicity of infection of 0.1 for 48 hours. MVA-H1-Ca or rVVL-H1-Ca infected cells were harvested by scraping or by adding trypsin. MVA-N1-Ca or rVVL-N1-Ca infected cells were harvested by scraping. Sonicated cell lysates were loaded onto 12% polyacrylamide gels and afterwards blotted on nitrocellulose membrane. To detect the H1 protein, a sheep antiserum against the A/California/7/2009 hemagglutinin (NIBSC 09/152) was used. Donkey-anti-sheep alkaline phosphatase-conjugated IgG was used as a secondary antibody. To detect the N1 protein ab21304 was utilized. Goat-anti-rabbit alkaline phosphatase-conjugated IgG was used as a secondary antibody. A whole virus vaccine H1N1 A/California/7/2009  served as positive control.
Neuraminidase expression in chicken cells.
ab21304 has been referenced in 5 publications.
- Fan RL et al. Generation of Live Attenuated Influenza Virus by Using Codon Usage Bias. J Virol 89:10762-73 (2015). WB . PubMed: 26269186
- Tripathi S et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis 4:e562 (2013). WB . PubMed: 23538443
- Hessel A et al. A pandemic influenza H1N1 live vaccine based on modified vaccinia Ankara is highly immunogenic and protects mice in active and passive immunizations. PLoS One 5: (2010). WB . PubMed: 20808939
- Takahashi T et al. The low-pH stability discovered in neuraminidase of 1918 pandemic influenza A virus enhances virus replication. PLoS One 5:e15556 (2010). Flow Cyt ; Influenza A . PubMed: 21151571
- Poon LL et al. Vaccinia virus-based multivalent H5N1 avian influenza vaccines adjuvanted with IL-15 confer sterile cross-clade protection in mice. J Immunol 182:3063-71 (2009). WB . PubMed: 19234203