The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 21151571
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration. It will detect 10 ng of free peptide at 1 µg/ml.
Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.
Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).
Western blot - Anti-Avian Influenza A Neuraminidase antibody (ab21304)Image from Hessel A et al, PLoS One. 2010 Aug 16;5(8). pii: e12217, Fig 1.
Expression of the H1 or N1 proteins by recombinant vaccinia viruses was detected by Western blotting. Vero cells in case of the VV-L constructs, or the avian cell line DF-1  in case of MVA, were infected at a multiplicity of infection of 0.1 for 48 hours. MVA-H1-Ca or rVVL-H1-Ca infected cells were harvested by scraping or by adding trypsin. MVA-N1-Ca or rVVL-N1-Ca infected cells were harvested by scraping. Sonicated cell lysates were loaded onto 12% polyacrylamide gels and afterwards blotted on nitrocellulose membrane. To detect the H1 protein, a sheep antiserum against the A/California/7/2009 hemagglutinin (NIBSC 09/152) was used. Donkey-anti-sheep alkaline phosphatase-conjugated IgG was used as a secondary antibody. To detect the N1 protein ab21304 was utilized. Goat-anti-rabbit alkaline phosphatase-conjugated IgG was used as a secondary antibody. A whole virus vaccine H1N1 A/California/7/2009  served as positive control. Neuraminidase expression in chicken cells.
Tripathi S et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis4:e562 (2013).
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