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Anti-Avian Influenza A Neuraminidase antibody (ab21305)

Submit a review Q&A (3)References (3)

Key features and details

  • Rabbit polyclonal to Avian Influenza A Neuraminidase
  • Suitable for: ELISA
  • Isotype: IgG

Overview

  • Product name

    Anti-Avian Influenza A Neuraminidase antibody
    See all Avian Influenza A Neuraminidase primary antibodies

  • Description

    Rabbit polyclonal to Avian Influenza A Neuraminidase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Synthetic peptide corresponding to 16 amino acids in the middle of the Neuraminidase protein. Efforts were made to use relatively conserved regions as the antigen.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Notes
Application notes
ELISA: Use at a concentration of 1 µg/ml, detects 10 ng of free peptide.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Relevance

    Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moities on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication.

  • Cellular localization

    Cell Membrane; Virion membrane. Apical cell membrane; Single-pass type II membrane protein (By similarity).

  • Alternative names

    • NA antibody
    • Neuraminidase antibody

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab21305? Please let us know so that we can cite the reference in this datasheet.

ab21305 has been referenced in 3 publications.

  • Wu CY  et al. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PLoS One 7:e42363 (2012). PubMed: 22879951
  • Wu CY  et al. Mammalian expression of virus-like particles for advanced mimicry of authentic influenza virus. PLoS One 5:e9784 (2010). WB, ICC/IF . PubMed: 20339535
  • Su CY  et al. In vitro evaluation of neuraminidase inhibitors using the neuraminidase-dependent release assay of hemagglutinin-pseudotyped viruses. Antiviral Res 79:199-205 (2008). PubMed: 18453004

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

After contacting with this customer, he provided the information as follows, He wants to know that ab21304 might recognize the strain A/WSN/1933(H1N1) virus ;NCBI GI 194352376 or not. http://www.ncbi.nlm.nih.gov/protein/194352376 1 mnpnqkiiti gsicmvvgii slilqignii siwishsiqt gnqnhtgicn qgsitykvva 61 gqdstsvilt gnsslcpirg waihskdngi rigskgdvfv irepfiscsh lecrtffltq 121 gallndkhsr gtfkdrspyr almscpvgea pspynsrfes vawsasachd gmgwltigis 181 gpddgavavl kyngiiteti kswrknilrt qesectcvng scftimtdgp sdglasykif 241 kiekgkvtks ielnapnshy eecscypdtg kvmcvcrdnw hgsnrpwvsf dqnldykigy 301 icsgvfgdnp rpkdgtgscg pvsadgangv kgfsykygng vwigrtksds srhgfemiwd 361 pngwtetdsr fsmrqdvvam tdrsgysgsf vqhpeltgld cmrpcfwvel irglpeenai 421 wtsgsiisfc gvnsdtvdws wpdgaelpft idk Could you please help this customer to check the alignment for him, because he will decide whether he buy or not. thank you very much！ Sincerely yours,

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Answer

Thank you for kindly providing the sequence. This has been checked, and has the following percentage alignment with the following two antibodies: ab21304 Avian Influenza A Neuraminidase antibody: 80% ab21305 Avian Influenza A Neuraminidase antibody: 87% We recommend alignment shoudl be over 85% to predict that an antibody will detect. Therefore, there is likely to be some crossreactivity with ab21304, but ab21305 may be a better choice based on the alignment scores. Please check the online datasheet to ensure this has been tested in the application the customer would like to use. Click here (or use the following: https://www.abcam.com/Avian-Influenza-A-Neuraminidase-antibody-ab21305.html). I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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Question

Dear Sir/Madam My name is Shenshen Cai, currently working at University of Pennsylvania on some antigenic peptide projects. I see some H5N1 antibodies in your catalog. May I know the sequences of the immunogenic peptides you used to raise your antibodies ab21292, ab21305, ab21297, ab21304? thank you very much, I look forward to hearing from you.

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Answer

Thank you for your enquiry. Unfortunately, the immunogen sequences for ab21292, ab21297, ab21304 and ab21305 are proprietary. However, I can give you a range from which we selected the peptides. ab21305 - residues 150-200 ab21304 – residues 400-450 ab21292 – residues 300-350 ab21297 – residues 100-150 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Could you please let us know whether this antibody can detect the NA protein of H5N1. We are looking forward to your replying. Thank you very much. Regards,

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Answer

Thank you for your enquiry and your interest in our products. This product should detect the protein. Unfortunately, we have only been able to test the antibody against the immunogen peptide in ELISA.

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