Product nameAnti-Avian Influenza Nucleoprotein antibody
See all Avian Influenza Nucleoprotein primary antibodies
DescriptionRabbit polyclonal to Avian Influenza Nucleoprotein
Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
Synthetic peptide, corresponding to amino acids 428-441 of Avian Influenza Nucleoprotein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab22285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.1 µg/ml.|
|WB||Use at an assay dependent concentration. PubMed: 23538443|
RelevanceEncapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
This product has been referenced in:
- Tripathi S et al. Influenza A virus nucleoprotein induces apoptosis in human airway epithelial cells: implications of a novel interaction between nucleoprotein and host protein Clusterin. Cell Death Dis 4:e562 (2013). WB . Read more (PubMed: 23538443) »
- Yamamoto Y et al. Detecting avian influenza virus (H5N1) in domestic duck feathers. Emerg Infect Dis 14:1671-2 (2008). Read more (PubMed: 18826847) »