Key features and details
- Rabbit polyclonal to Avian Influenza Nucleoprotein
- Suitable for: WB
- Isotype: IgG
Product nameAnti-Avian Influenza Nucleoprotein antibody
See all Avian Influenza Nucleoprotein primary antibodies
DescriptionRabbit polyclonal to Avian Influenza Nucleoprotein
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Other species
- Recombinant fusion protein of avian flu nucleoprotein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab25921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Please note from the image legend that a recombinant fusion protein was used to generate the western blot image.
The molecular weight shown is the molecular weight of the fusion protein (e.g nucleoprotein amino acids 58-77 + fusion protein) not the Avian Influenza Nucleoprotein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceEncapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus
- NP antibody
- Nucleocapsid protein antibody
- Nucleoprotein antibody
- Protein N antibody
All lanes : Anti-Avian Influenza Nucleoprotein antibody (ab25921) at 0.5 µg/ml
Lane 1 : Recombinant fusion protein containing amino acids 58-77 of Avian flu nucleoprotein at 0.1 µg
Lane 2 : Fusion partner without these amino acids
Observed band size: 31 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa, 59 kDa, 66 kDa. We are unsure as to the identity of these extra bands.
ab25921 has not yet been referenced specifically in any publications.