Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Chicken
  • Conjugation
    HRP

Specifications

Our Abpromise guarantee covers the use of ab59653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Western blot

    ELISA

  • Form
    Liquid
  • Additional notes


    Avidin-HRP conjugates are useful for detection, identification, quantitation, and localization of biotinylated substances.

    Reacts with biotinylated substances.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituents: 0.1% BSA, PBS

General Info

  • Alternative names
    • AVD
    • AVID_CHICK
    • Avidin
    • LOC396260
  • Function
    The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
  • Tissue specificity
    Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
  • Sequence similarities
    Belongs to the avidin/streptavidin family.
    Contains 1 avidin-like domain.
  • Post-translational
    modifications
    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
  • Cellular localization
    Secreted.
  • Information by UniProt

References

ab59653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and you rinterest in our products.

I can confirm that ab6675 has been tested in indirect ELISA; we do not have any data if it works together with ab59653 in Sandwich ELISA (sELISA) system. Unfortunately, we do not have any supporting data yet. However, ab6675 will work with a compatible secondary antibody.

I have conducted a search on our website and found a few antibodies for Avidin but none of them have been characterized for sELISA.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up