Product nameAnti-AVPR1B antibody
See all AVPR1B primary antibodies
DescriptionRabbit polyclonal to AVPR1B
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Mouse AVPR1B aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane
This product was previously labelled as AVPR1B, Vasopressin V1b receptor
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab104365 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionReceptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- Antidiuretic hormone receptor 1b antibody
- Antidiuretic hormone, receptor for, V1B antibody
- Arginine vasopressin receptor 1B antibody
All lanes : Anti-AVPR1B antibody (ab104365) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
VR1b contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ICC/IF image of ab104365 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) SKNSH cells at 1µg/ml.
ab104365 has not yet been referenced specifically in any publications.