Overview

  • Product name

  • Description

    Rabbit polyclonal to AVPR1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse AVPR1B aa 200-300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab124133)

  • Positive control

    • This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane
  • General notes

     This product was previously labelled as AVPR1B, Vasopressin V1b receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab104365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function

    Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antidiuretic hormone receptor 1b antibody
    • Antidiuretic hormone, receptor for, V1B antibody
    • Arginine vasopressin receptor 1B antibody
    • Arginine vasopressin receptor 3 antibody
    • AVPR V1b antibody
    • AVPR V3 antibody
    • Avpr1b antibody
    • AVPR3 antibody
    • Pituitary vasopressin receptor 3 antibody
    • V1bR antibody
    • V1BR_HUMAN antibody
    • Vasopressin V1b receptor antibody
    • Vasopressin V3 receptor antibody
    • VPR3 antibody
    see all

Images

  • All lanes : Anti-AVPR1B antibody (ab104365) at 1 µg/ml

    Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
    Lane 2 : Forebrain (Mouse) Tissue Lysate
    Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
    Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 102 kDa, 22 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    VR1b contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab104365 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104365, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) SKNSH cells at 1µg/ml.

References

ab104365 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 30min in citrate buffer @ 90oC
Specification
Brain
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Emma Schofield

Verified customer

Submitted Sep 15 2016

Answer

Thank you for your inquiry.

I am happy to confirm that the immunogen of ab104365 shows less than 15% homology with VR1alpha receptor (3 residues out of 22).

According to this data it is extremely unlikely ab104365 is binding to VR1alpha receptor.

I would like to suggest to investigate other reason for differing expression levels on RNA and protein level like protein half-life etc.

I am sorry I could not help solve that puzzle and hope this information is nevertheless helpful.

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