Overview

  • Product name

    Anti-AWP1 antibody [EPR12323]
    See all AWP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12323] to AWP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human AWP1 (internal sequence). The exact sequence is proprietary.
    Database link: Q6FIF0

  • Positive control

    • Jurkat, HepG2, A673 cell lysates. Permeabilized Jurkat cells.
  • General notes

     This product was previously labelled as ZA20D3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12323
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-AWP1 antibody [EPR12323] (ab180856) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa
      Observed band size: 26 kDa
      why is the actual band size different from the predicted?

    • Flow cytometry analysis of AWP1 expression in permeabilized Jurkat cells using ab180856 at 1/10 dilution (red) and a rabbit IgG as negative control (green).

    References

    ab180856 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab180856.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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