Recombinant Anti-AWP1 antibody [EPR12323] (ab180856)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12323] to AWP1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AWP1 antibody [EPR12323]
See all AWP1 primary antibodies -
Description
Rabbit monoclonal [EPR12323] to AWP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human AWP1 (internal sequence). The exact sequence is proprietary.
Database link: Q6FIF0 -
Positive control
- Jurkat, HepG2, A673 cell lysates. Permeabilized Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12323 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
Target
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Relevance
ZA20D3 is a zinc-finger protein located on chromosome 15. It is associated with PRK1 -
Database links
- Entrez Gene: 54469 Human
- Entrez Gene: 65098 Mouse
- Entrez Gene: 293067 Rat
- Omim: 610183 Human
- SwissProt: Q6FIF0 Human
- SwissProt: Q9BQF7 Human
- SwissProt: Q9DCH6 Mouse
- SwissProt: Q6DGF4 Rat
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Alternative names
- 3110005P07Rik antibody
- AN1-type zinc finger protein 6 antibody
- Associated with PRK1 protein antibody
see all
Images
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All lanes : Anti-AWP1 antibody [EPR12323] (ab180856) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of AWP1 expression in permeabilized Jurkat cells usingab180856 at 1/10 dilution (red) and a rabbit IgG as negative control (green).
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180856 has not yet been referenced specifically in any publications.