- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Axin 1 antibody
See all Axin 1 primary antibodies -
DescriptionGoat polyclonal to Axin 1
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Host speciesGoat
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Zebrafish, Baboon, Rhesus monkey
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Immunogen
Synthetic peptide:
FEEKIIGKVEKVD
(with a Cysteine residue linker), corresponding to C terminal amino acids 850-862 of Human Axin 1 -
Positive control
- Human adrenal tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab115205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionComponent of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
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Tissue specificityUbiquitously expressed.
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Involvement in diseaseHepatocellular carcinoma
Caudal duplication anomaly -
Sequence similaritiesContains 1 DIX domain.
Contains 1 RGS domain. -
DomainThe tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
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Post-translational
modificationsPhosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
Cellular localizationCytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
- Information by UniProt
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Database links
- Entrez Gene: 395786 Chicken
- Entrez Gene: 504357 Cow
- Entrez Gene: 100065116 Horse
- Entrez Gene: 8312 Human
- Entrez Gene: 12005 Mouse
- Entrez Gene: 79257 Rat
- Entrez Gene: 57931 Zebrafish
- Omim: 603816 Human
see all -
Alternative names
- AI316800 antibody
- AXIN antibody
- Axin 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)ab114205, at 3.75 ug/ml, staining Axin-1 in formalin-fixed, paraffin-embedded Human adrenal tissue by immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of rat bladder tissue, staining Axin 1 with ab115205.
Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.
Protocols
References
ab115205 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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