For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    axin-1-antibody-ab115205.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Cell Cycle Inhibitors Other
Share by email

Anti-Axin 1 antibody (ab115205)

  • Datasheet
Reviews (1) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)

Key features and details

  • Goat polyclonal to Axin 1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Primary
Product image
Anti-Axin 2 antibody (ab107613)
Primary
Product image
Anti-Axin 2 antibody [EPR2005(2)] (ab109307)
Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-Axin 1 antibody
    See all Axin 1 primary antibodies
  • Description

    Goat polyclonal to Axin 1
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    FEEKIIGKVEKVD

    (with a Cysteine residue linker), corresponding to C terminal amino acids 850-862 of Human Axin 1
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human adrenal tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human Axin 1 protein (ab204090)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab115205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
All applications
Mouse
Rat
Horse
Chicken
Cow
Zebrafish
Baboon
Rhesus monkey
Application Abreviews Notes
IHC-P (1)
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IHC-P
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
  • Tissue specificity

    Ubiquitously expressed.
  • Involvement in disease

    Hepatocellular carcinoma
    Caudal duplication anomaly
  • Sequence similarities

    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • Domain

    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    modifications

    Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • Cellular localization

    Cytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
  • Target information above from: UniProt accession O15169 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395786 Chicken
    • Entrez Gene: 504357 Cow
    • Entrez Gene: 100065116 Horse
    • Entrez Gene: 8312 Human
    • Entrez Gene: 12005 Mouse
    • Entrez Gene: 79257 Rat
    • Entrez Gene: 57931 Zebrafish
    • Omim: 603816 Human
    • SwissProt: O42400 Chicken
    • SwissProt: O15169 Human
    • SwissProt: O35625 Mouse
    • SwissProt: O70239 Rat
    • SwissProt: P57094 Zebrafish
    • Unigene: 592082 Human
    • Unigene: 23684 Mouse
    • Unigene: 31781 Rat
    • Unigene: 80549 Zebrafish
    see all
  • Alternative names

    • AI316800 antibody
    • AXIN antibody
    • Axin 1 antibody
    • Axin-1 antibody
    • axin1 antibody
    • AXIN1_HUMAN antibody
    • Axis inhibition protein 1 antibody
    • Axis Inhibitor 1 antibody
    • Fu antibody
    • fused antibody
    • Fused, mouse, homolog of antibody
    • hAxin antibody
    • Kb antibody
    • Ki antibody
    • kinky antibody
    • knobbly antibody
    • MGC132911 antibody
    • MGC52315 antibody
    • PPP1R49 antibody
    • Protein Fused antibody
    • Protein phosphatase 1 regulatory subunit 49 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)
    ab114205, at 3.75 ug/ml, staining Axin-1 in formalin-fixed, paraffin-embedded Human adrenal tissue by immunohistochemistry.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 1 antibody (ab115205)This image is courtesy of an anonymous Abreview
    Immunohistochemical analysis of rat bladder tissue, staining Axin 1 with ab115205.

    Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®488-conjugated donkey anti-goat polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab115205? Please let us know so that we can cite the reference in this datasheet.

    ab115205 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Axin 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Bladder)
    Specification
    Bladder
    Fixative
    10% Buffered Formalin
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: .01M Citrate Buffer Ph 6.0
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5000µg/mL · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.