Overview

  • Product name
  • Description
    Goat polyclonal to Axin 1
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide: FEEKIIGKVEKVD, corresponding to C terminal amino acids 850-862 of Human AXIN1.

  • General notes
    Principal Names - AXIN1; AXIN; MGC52315; axin 1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1 Official Gene Symbol - AXIN1 GenBank Accession Number – NP_003493; NP_851393 LocusLink ID - 8312 (human); 12005 (mouse); 79257 (rat) Gene Ontology terms - signal transducer activity; oocyte axis determination; frizzled receptor signaling pathway; development; apoptosis; cytoplasm; negative regulation of cell cycle.

Properties

Applications

Our Abpromise guarantee covers the use of ab4157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Can be blocked with Human Axin 1 peptide (ab23000). No signal obtained yet but low background observed in Human Brain, Human Pancreas, MOLT-4 and HeLa lysates at up to 2.0µg/ml.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
  • Tissue specificity
    Ubiquitously expressed.
  • Involvement in disease
    Hepatocellular carcinoma
    Caudal duplication anomaly
  • Sequence similarities
    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • Domain
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    modifications
    Phosphorylation and dephosphorylation of AXIN1 regulates assembly and function of the beta-catenin complex. Phosphorylated by CK1 and GSK3B. Dephosphorylated by PPP1CA and PPP2CA. Phosphorylation by CK1 enhances binding of GSK3B to AXIN1.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination at 'Lys-48' and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Sumoylation at Lys-857 and Lys-860 prevents ubiquitination and degradation. Sumoylation is required for AXIN1-mediated JNK activation. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important for nuclear accumulation during Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • Cellular localization
    Cytoplasm. Nucleus. Membrane. Cell membrane. MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684).
  • Information by UniProt
  • Database links
  • Alternative names
    • AI316800 antibody
    • AXIN antibody
    • Axin 1 antibody
    • Axin-1 antibody
    • axin1 antibody
    • AXIN1_HUMAN antibody
    • Axis inhibition protein 1 antibody
    • Axis Inhibitor 1 antibody
    • Fu antibody
    • fused antibody
    • Fused, mouse, homolog of antibody
    • hAxin antibody
    • Kb antibody
    • Ki antibody
    • kinky antibody
    • knobbly antibody
    • MGC132911 antibody
    • MGC52315 antibody
    • PPP1R49 antibody
    • Protein Fused antibody
    • Protein phosphatase 1 regulatory subunit 49 antibody
    see all

Images

  • All lanes : Anti-Axin 1 antibody (ab4157) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
    Lane 2 : Jurkat whole cell lysate (ab7899)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat IgG secondary antibody (ab28445) at 1/10000 dilution
  • ab4157 (3.8 µg/ml) staining Azin 1 in Human adrenal cortex by IHC-P (Immunohistochemistry of paraffin-embedded sections). Steamed antigen retrievalwith citrate buffer (pH 6) was performed. Image shows membranous and fibrous staining in and around the secretory cells of the zona fasciculata in the cortex.

References

ab4157 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for youe email, I'm glad to hear that this antibody appears to be working for you. We value all feedback from researchers and since this is a Fast Track antibody, it is especially important. Once you have more information, please submit feedback to fast-track@abcam.com We will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including an image and details of materials & methods). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with $300 of Conference Travel Money. Other useful positive and negative feedback will be rewarded with a $15 (£10/€15) Amazon gift certificate. All awards are subject to approval by Abcam scientists and provision of suitable data. If you have any more questions, please do contact me again.

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Question
Answer

Thank you so much for your patience! :) As stated on the online datasheet, ab4157 is a "fast track" antibody and thus far, no signal has been obtained but low background was observed in Human Brain, Human Pancreas, MOLT-4 and HeLa lysates at up to 2.0µg/ml. We have had no customer feedback yet regarding this antibody and we greatly appreciate your comments and information about your results. Also, we're awarding $300 Conference Travel Money to the 1st researcher who submits positive or conclusive negative data, more details are located on the online datasheet. I'm currently in contact with the antibody's originator to see if they have any suggestions for you and some more information from you would help us out. What type of samples are you using? At what dilution are you using the primary at? What is your secondary antibody? At approximately what size do you see the band? Thank you, and I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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