• Product name
  • Description
    Rabbit polyclonal to Axin 2
  • Host species
  • Specificity
    ab107613 antibody is predicted to not cross-react with Axin 1. At least four isoforms of Axin 2 are known to exist; this antibody will recognize all four.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Axin 2 (C terminal).

  • Positive control
    • Mouse lung tissue lysate. This antibody gave a positive result in IF/ICC when used in the following methanol fixed cell lines: PC12.



Our Abpromise guarantee covers the use of ab107613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa.


  • Function
    Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
  • Tissue specificity
    Expressed in brain and lymphoblast.
  • Involvement in disease
    Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
    Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
  • Sequence similarities
    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • Domain
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Axil antibody
    • Axin like protein antibody
    • Axin-2 antibody
    • Axin-like protein antibody
    • Axin2 antibody
    • AXIN2_HUMAN antibody
    • Axis inhibition protein 2 antibody
    • Conductin antibody
    • DKFZp781B0869 antibody
    • MGC10366 antibody
    • MGC126582 antibody
    see all


  • Immunohistochemical analysis of Axin 2 expression in mouse lung tissue with ab107613 at 20µg/ml.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse lung tissue staining Axin 2 with ab107613 at 20µg/ml
  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse lung tissue labeling Axin 2 with Anti-Axin 2 antibody (ab107613) at 5μg/ml.
  • Anti-Axin 2 antibody (ab107613) at 1 µg/ml + Mouse lung tissue lysate at 15 µg

    Predicted band size: 94 kDa

  • Immunohistochemistry of AXIN2 in rat lung tissue with ab107613 at 5 µg/ml
  • ICC/IF image of ab107613 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107613 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab107613 staining Axin 2 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/800) was used as the secondary antibody.

    See Abreview


This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

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Verified customer

Submitted Feb 12 2013

As requested I have issued a free of charge replacement with one unit of ab107613, the order number is *******.

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