Product nameAnti-Axin 2 antibody
See all Axin 2 primary antibodies
DescriptionRabbit polyclonal to Axin 2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human Axin 2 aa 1-843.
MSSAMLVTCLPDPSSSFREDAPRPPVPGEEGETPPCQPGVGKGQVTKPMS VSSNTRRNED GLGEPEGRASPDSPLTRWTKSLHSLLGDQDGAYLFRTF LEREKCVDTLDFWFACNGFRQM NLKDTKTLRVAKAIYKRYIENNSIVS KQLKPATKTYIRDGIKKQQIDSIMFDQAQTEIQS VMEENAYQMFLTSD IYLEYVRSGGENTAYMSNGGLGSLKVVCGYLPTLNEEEEWTCADFK CK LSPTVVGLSSKTLRATASVRSTETVDSGYRSFKRSDPVNPYHIGSGYVFA PATSANDS EISSDALTDDSMSMTDSSVDGIPPYRVGSKKQLQREMHRS VKANGQVSLPHFPRTHRLPK EMTPVEPATFAAELISRLEKLKLELESR HSLEERLQQIREDEEREGSELTLNSREGAPTQ HPLSLLPSGSYEEDPQ TILDDHLSRVLKTPGCQSPGVGRYSPRSRSPDHHHHHHSQYHSL LPPG GKLPPAAASPGACPLLGGKGFVTKQTTKHVHHHYIHHHAVPKTKEEIEAE ATQRVH CFCPGGSEYYCYSKCKSHSKAPETMPSEQFGGSRGSTLPKRN GKGTEPGLALPAREGGAP GGAGALQLPREEGDRSQDVWQWMLESERQS KPKPHSAQSTKKAYPLESARSSPGERASRH HLWGGNSGHPRTTPRAHL FTQDPAMPPLTPPNTLAQLEEACRRLAEVSKPPKQRCCVASQ QRDRNH SATVQTGATPFSNPSLAPEDHKEPKKLAGVHALQASELVVTYFFCGEEIP YRRM LKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWED ETVLPMYEGRILGKVE RID
Database link: Q9Y2T1
- Mouse lung, mouse brain, mouse heart, mouse liver lysates
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 94 kDa.|
|IHC-P||1/50 - 1/200.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionInhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
Tissue specificityExpressed in brain and lymphoblast.
Involvement in diseaseDefects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
Sequence similaritiesContains 1 DIX domain.
Contains 1 RGS domain.
DomainThe tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
modificationsProbably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
- Information by UniProt
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185821. Blue DAPI for nuclear staining.
All lanes : Anti-Axin 2 antibody (ab185821) at 1/500 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse heart lysate
Lane 4 : Mouse liver lysate
Predicted band size: 94 kDa
ab185821 has not yet been referenced specifically in any publications.