Anti-Axin 2 antibody (ab218023)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Axin 2 antibody
See all Axin 2 primary antibodies -
Description
Rabbit polyclonal to Axin 2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human
-
Immunogen
Synthetic peptide within Human Axin 2 aa 350-400 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:PHFPRTHRLPK EMTPVEPATF AAELISRLEK LKLELESRHS LEERLQQIRE
Database link: Q9Y2T1 -
Positive control
- Rat brain and rat lymph node tissues.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab218023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. |
Target
-
Function
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B. -
Tissue specificity
Expressed in brain and lymphoblast. -
Involvement in disease
Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types. -
Sequence similarities
Contains 1 DIX domain.
Contains 1 RGS domain. -
Domain
The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2. -
Post-translational
modificationsProbably phosphorylated by GSK3B and dephosphorylated by PP2A.
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 8313 Human
- Entrez Gene: 12006 Mouse
- Entrez Gene: 29134 Rat
- Omim: 604025 Human
- SwissProt: Q9Y2T1 Human
- SwissProt: O88566 Mouse
- SwissProt: O70240 Rat
- Unigene: 156527 Human
see all -
Alternative names
- Axil antibody
- Axin like protein antibody
- Axin-2 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab218023)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat lymph node tissue labeling Axin 2 with ab218023 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Axin 2 antibody (ab218023)Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling Axin 2 with ab218023 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Protocols
Datasheets and documents
References
ab218023 has not yet been referenced specifically in any publications.
