Overview

  • Product name
  • Description
    Rabbit polyclonal to Axin 2
  • Host species
    Rabbit
  • Specificity
    Milk blocking not recommended
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse Axin 2.

    (Peptide available as ab32196.)

  • Positive control
    • This antibody gave a positive signal in: SW480, HCT 116, LS147T whole cell lysates; mouse colon tissue lysates and AXIN2 partial recombinant tagged protein

Properties

Applications

Our Abpromise guarantee covers the use of ab32197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Can be blocked with Mouse Axin 2 peptide (ab32196). Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa) in endogenous lysates. Ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.
Flow Cyt 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B.
  • Tissue specificity
    Expressed in brain and lymphoblast.
  • Involvement in disease
    Defects in AXIN2 are involved in colorectal cancer (CRC) [MIM:114500]. They appear to be specifically associated with defective mismatch repair.
    Defects in AXIN2 are the cause of oligodontia-colorectal cancer syndrome (ODCRCS) [MIM:608615]. Affected individuals manifest severe tooth agenesis and colorectal cancer or precancerous lesions of variable types.
  • Sequence similarities
    Contains 1 DIX domain.
    Contains 1 RGS domain.
  • Domain
    The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.
  • Post-translational
    modifications
    Probably phosphorylated by GSK3B and dephosphorylated by PP2A.
    ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.
    Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Axil antibody
    • Axin like protein antibody
    • Axin-2 antibody
    • Axin-like protein antibody
    • Axin2 antibody
    • AXIN2_HUMAN antibody
    • Axis inhibition protein 2 antibody
    • Conductin antibody
    • DKFZp781B0869 antibody
    • MGC10366 antibody
    • MGC126582 antibody
    see all

Images

  • Anti-Axin 2 antibody (ab32197) at 1 µg/ml + AXIN2 partial recombinant tagged protein at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 94 kDa



    ab32197 gave a positive signal against the tagged partial recombinant protein which has an expected molecular weight of 37 kDa.

  • ab32197 staining Axin2 in mouse colon sections. Secondary antibody conjugated to Alexa-fluor ® 488 (please see Abreview for further details)

    See Abreview

  • IHC image of ab32197 stained area of normal tissue in a human colorectal cancer FFPE section. Section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 seconds at 125°C. Section was incubated with ab32197 at a dilution of 1:500 for 1h at room temperature and detected using an HRP conjugated polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-Axin 2 antibody (ab32197) at 1 µg/ml

    Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : Colon (Mouse) Tissue Lysate
    Lane 3 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 4 : LS147T (Colon cancer cells) (Human) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 94 kDa
    Observed band size: 94 kDa
    Additional bands at: 32 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • Flow Cytometry analysis of human permeabilised REH B cells labeling Axin 2 with ab32197 (red). Cells were fixed with paraformaldehyde and permeabilized with saponin, followed by incubation with 2ul of undiluted Anti-Axin 2 antibody (ab32197)  in 100µl of cells in PBS for 15 minutes at 4°C. An undiluted polyclonal goat anti-rabbit Alexa Fluor® 488 was used as the secondary antibody. Control - rabbit IgG (white).

    See Abreview

References

This product has been referenced in:
  • Ding Y  et al. Deubiquitinase inhibitor b-AP15 activates endoplasmic reticulum (ER) stress and inhibits Wnt/Notch1 signaling pathway leading to the reduction of cell survival in hepatocellular carcinoma cells. Eur J Pharmacol 825:10-18 (2018). Read more (PubMed: 29454609) »
  • Yuan L & Tian J LIN28B promotes the progression of colon cancer by increasing B-cell lymphoma 2 expression. Biomed Pharmacother 103:355-361 (2018). WB ; Human . Read more (PubMed: 29669301) »

See all 31 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry which has been forwarded to the scientific support team. I can confirm that there was indeed an error and the datasheet has been amended by our datasheets team. I sincerely apologise for any confusion.

The quality ...

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Thank you very much for these precisions.

I would recommend to use 5% BSA as blocking agent. This is what is used in our lab to characterise the antibodies. We also use this blocking solution to dilute both primary and secondary antibodies. <...

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Thanks for sending along the sequence.

Unfortunately the alignment results show there is no sequence similarity between the peptide immunogen used to produce ab32197 and canine Axin2. So I don't think this antibody will cross react with your ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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I am unable to send you both antibodies as replacements but what I can do is send you one as a free replacement and also, as whichever one you receive has not been tested in IHC, I can also send a testing discount code. What that means is that you test...

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We do not have sample sizes of our antibodies, but I would be happy to send you a vial of either ab107613 or ab109307 free of charge, so that can test it out in IHC.

We have two other Axin2 antibodies in our catalogue, ab107613 an ab109307. Neither has been tested in IHC but both are guaranteed to work in western blot. If you would like to test either of these then please let me know, otherwise I will process your ...

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Thank you for contacting Abcam. I have look over the information and image you sent and I was wondering if you could provide a few more details please. Could you confirm the labeling of the image you sent, that lanes 3 & 5 are overexpressing the contro...

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Thank you for your phone call. DISCOUNT CODE: xxx Expiration date: xxx I am very pleased to hear you would like to accept our offer and test ab32197 in chicken. This code will give you 1 free PRIMARY ANTIBODY before the expiration da...

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Thank you for your enquiry. As you may know, ab32179 is one of our Fast-track antibodies and it is not yet fully characterized. I would therefore try a large range of dilutions in a titration experiment. For example, try 1:10, 1:100, 1:200, 1:500 and 1...

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1-10 of 11 Q&A

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