Western blot abreview for Anti-Axin 2 antibody [EPR2005(2)]

Excellent
Abreviews
Application
Western blot
Sample
Pig Cell lysate - whole cell (PAE cell line (Pig Aortic Endothelial))
Loading amount
70 µg
Specification
PAE cell line (Pig Aortic Endothelial)
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS T Milk 1%

Secondary antibody

Name
Non-Abcam antibody was used: DAKO anti Rb IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 80 kDa
Positive control
mouse 293T cell line

Mr. Raul Peña

Verified customer

Submitted Dec 17 2012

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