Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Axin 2 antibody [EPR2005(2)]

Average
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB-231 breast cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
No
Specification
MDA-MB-231 breast cancer xenograft
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS
Dilution
1/150

Secondary antibody

Dilution
1/600
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647

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Submitted Dec 14 2015

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