Overview

  • Product name

  • Description

    Rabbit polyclonal to Axl
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Recombinant fragment within Human Axl (internal sequence). The exact sequence is proprietary.
    Database link: P30530

  • Positive control

    • WB: HEK-293T cells expressing recombinant human Axl; H1299 and A549 whole cell lysates. IHC-P: Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab226927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 98 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity

    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Images

  • Paraffin-embedded human colon carcinoma tissue stained for Axl with ab226927 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Axl antibody (ab226927) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T transfected with human Axl, whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 98 kDa



    7.5% SDS-PAGE

  • All lanes : Anti-Axl antibody (ab226927) at 1/500 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 98 kDa



    5% SDS-PAGE

References

ab226927 has not yet been referenced specifically in any publications.

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