Overview

  • Product name

  • Description

    Rabbit polyclonal to Axl
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Axl (internal sequence). The exact sequence is proprietary.
    Database link: P30530

  • Positive control

    • WB: HEK-293 transfected with Axl whole cell lysate; A549, NCI-H1299 and HCT 116 whole cell extracts; Mouse muscle tissue extract. IHC-P: Human non-small cell lung carcinoma, colon carcinoma and lung cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab227871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Predicted molecular weight: 98 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity

    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Images

  • All lanes : Anti-Axl antibody (ab227871) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with Axl, whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 98 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human lung cancer tissue stained for Axl using ab227871 at 1/200 dilution in immunohistochemical analysis. The signal was developed using goat anti-rabbit IgG antibody (Dylight®488) (green).

    Nuclear counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded human colon carcinoma tissue stained for Axl using ab227871 at 1/500 dilution in immunohistochemical analysis.

  • Anti-Axl antibody (ab227871) at 1/500 dilution + Mouse muscle tissue extract at 50 µg

    Predicted band size: 98 kDa



    5% SDS-PAGE gel.

  • All lanes : Anti-Axl antibody (ab227871) at 1/500 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 98 kDa



    5% SDS-PAGE gel.

  • Paraffin-embedded human non-small cell lung carcinoma tissue stained for Axl using ab227871 at 1/500 dilution in immunohistochemical analysis.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C

Abcam user community

Verified customer

Submitted Sep 10 2019

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