Anti-Axl antibody (ab32828)

Rabbit polyclonal Axl antibody. Validated in WB and tested in Mouse, Rat, Human, Monkey. Cited in 1 publication(s). Immunogen corresponding to synthetic peptide.

Overview

  • Product name

  • Description

    Rabbit polyclonal to Axl
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide:

    DSCSCLTAAEVHPAGRYVLCPS

    , corresponding to C terminal amino acids 843-864 of Human Axl. The peptide was post translationaly modified to achieve proper orientation and antigenicity.

  • Positive control

    • Rat testis extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab32828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 98 kDa.

Target

  • Function

    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity

    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

References

This product has been referenced in:

  • Zhang M  et al. Jujuboside A promotes Aß clearance and ameliorates cognitive deficiency in Alzheimer's disease through activating Axl/HSP90/PPAR? pathway. Theranostics 8:4262-4278 (2018). Read more (PubMed: 30128052) »
  • Guo Z  et al. Axl inhibition induces the antitumor immune response which can be further potentiated by PD-1 blockade in the mouse cancer models. Oncotarget 8:89761-89774 (2017). WB ; Mouse . Read more (PubMed: 29163786) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (Macaca fascicularis treated with MPTP brain sectio)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% NaBH4 in [0.05M] PBS for 30minutes at room temperature
Specification
Macaca fascicularis treated with MPTP brain sectio
Blocking step
2% donkey serum, 0.05% gelatin, 0.3% Trinton-X-100 in [0.05M] PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Perfused 4% PFA + 0.1% Glutaraldehyde and post-fixed 24h in 4% PFA

Abcam user community

Verified customer

Submitted Mar 07 2019

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