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Rabbit polyclonal to Axl
Predicted to work with:
Non human primates
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 Axl.
(Peptide available as
This antibody gave a positive signal in the following human tissue lysates:
Heart; Skeletal Muscle; Liver; Brain
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 98 kDa).
May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Information by UniProt
Adhesion related kinase antibody
Western blot - Anti-Axl antibody (ab77773)
All lanes :
Anti-Axl antibody (ab77773) at 1 µg/ml
Lane 1 :
Human heart tissue lysate - total protein (
Lane 2 :
Human skeletal muscle tissue lysate - total protein (
Lane 3 :
Human liver tissue lysate - total protein (
Lane 4 :
Human brain tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
107 kDa (
why is the actual band size different from the predicted?
Additional bands at:
70 kDa. We are unsure as to the identity of these extra bands.
Axl contains a number of potential phosphorylation and glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"