Overview

  • Product name

    Anti-Axl antibody [EPR19880]
    See all Axl primary antibodies
  • Description

    Rabbit monoclonal [EPR19880] to Axl
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Axl aa 1-250. The exact sequence is proprietary.
    Database link: P30530

  • Positive control

    • WB: 293T transfected with a His-tagged human Axl construct whole cell lysate. IHC-P: Human breast and breast carcinoma, lung and lung carcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab219651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000. Predicted molecular weight: 98 kDa.

Detection of endogenous expression of AXL in WB will need optimization.  Under our experimental conditions we could not detect endogenous AXL in human cell and tissue lysates.

Target

  • Function

    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity

    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast and breast carcinoma tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on cancer cells of the human breast carcinoma, and the pericarcinous tissue was nearly negative (magnification x150) (PMID: 25337673).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining on human lung.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on cancer cells of human lung carcinoma (PMID: 25337673).

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human adipose tissue labeling Axl with ab219651 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Negative control: No staining on human adipose tissues (PMID: 26573603).

    Counter stained with Hematoxylin.

     

  • All lanes : Anti-Axl antibody [EPR19880] (ab219651) at 1/10000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney) transfected with an empty expression vector, whole cell lysate
    Lane 2 : 293T (Human epithelial cell line from embryonic kidney) transfected with a His-tagged human Axl construct whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/100000 dilution

    Predicted band size: 98 kDa
    Observed band size: 138 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Detection of endogenous expression of Axl in WB will need optimization. Under our experimental conditions we could not detect endogenous Axl in human cell and tissue lysates.

References

ab219651 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab219651.
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