Recombinant
RabMAb

Recombinant Anti-Axl antibody [EPR21107] (ab215205)

Overview

  • Product name

    Anti-Axl antibody [EPR21107]
    See all Axl primary antibodies
  • Description

    Rabbit monoclonal [EPR21107] to Axl
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Axl aa 1-250. The exact sequence is proprietary.
    Database link: P30530

  • Positive control

    • WB: DU 145, NCI-H1299, 4T1, C2C12, HeLa and HUVEC whole cell lysates; Human skeletal muscle lysate; Rat and mouse muscle lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 80, 140 kDa (predicted molecular weight: 98 kDa).

Target

  • Function

    May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
  • Tissue specificity

    Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adhesion related kinase antibody
    • AI323647 antibody
    • Ark antibody
    • Axl antibody
    • AXL oncogene antibody
    • AXL receptor tyrosine kinase antibody
    • AXL transforming gene antibody
    • AXL transforming sequence/gene antibody
    • EC 2.7.10.1 antibody
    • JTK11 antibody
    • Oncogene AXL antibody
    • Tyro7 antibody
    • Tyrosine protein kinase receptor UFO antibody
    • Tyrosine-protein kinase receptor UFO antibody
    • UFO antibody
    • UFO_HUMAN antibody
    see all

Images

  • All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

    Lane 1 : DU 145 (human prostate carcinoma cell line) whole cell lysate
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 98 kDa
    Observed band size: 140,80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 29 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Jurkat
    The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).

    The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).

  • All lanes : Anti-Axl antibody [EPR21107] (ab215205) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate at 20 µg
    Lane 2 : Rat muscle lysate at 10 µg
    Lane 3 : Mouse muscle lysate at 10 µg
    Lane 4 : 4T1 (mouse mammary gland carcinoma cell line) whole cell lysate at 20 µg
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell lysate at 20 µg
    Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
    Lane 7 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 98 kDa
    Observed band size: 140,80 kDa why is the actual band size different from the predicted?



    Exposure time : Lane 1: 6 seconds; Lane 2: 3 seconds; Lane 3: 46 seconds; Lanes 4-5: 3 minutes; Lanes 6-7: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The 140 kDa band corresponds to the full-length Axl, while the 80 kDa band could be the cleaved form of Axl (PMID: 7822279 and 19541935).

    The apparent molecular mass is higher than predicted, likely due to glycosylation (PMID: 23629654).

References

ab215205 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
IHC - Wholemount
Sample
Mouse Tissue (Tumor)
Specification
Tumor

Abcam user community

Verified customer

Submitted Aug 20 2018

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