Key features and details
- Mouse monoclonal [MM0098-2N33] to Axl
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Axl antibody [MM0098-2N33]
See all Axl primary antibodies
DescriptionMouse monoclonal [MM0098-2N33] to Axl
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Human recombinant Axl (extracellular region).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein G purified
Purification notesThe IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
Our Abpromise guarantee covers the use of ab89224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
FunctionMay function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor.
Tissue specificityHighly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
- Information by UniProt
- Adhesion related kinase antibody
- AI323647 antibody
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ab89224 staining Axl in african green monkey CV1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde and then blocked using 5% serum for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 546 (red) used at a 1/400 dilution. DAPI was used to counterstain nuclei.
ab89224 has been referenced in 5 publications.
- Kong L et al. Qigesan reduces the motility of esophageal cancer cells via inhibiting Gas6/Axl and NF-?B expression. Biosci Rep 39:N/A (2019). PubMed: 31110076
- Koopman LA et al. Enapotamab vedotin, an AXL-specific antibody-drug conjugate, shows preclinical antitumor activity in non-small cell lung cancer. JCI Insight 4:N/A (2019). PubMed: 31600169
- Kwon JY et al. Relevance of placental type I interferon beta regulation for pregnancy success. Cell Mol Immunol N/A:N/A (2018). PubMed: 29907882
- Antony J et al. The tumour suppressor OPCML promotes AXL inactivation by the phosphatase PTPRG in ovarian cancer. EMBO Rep 19:N/A (2018). PubMed: 29907679
- Kong X et al. Cancer drug addiction is relayed by an ERK2-dependent phenotype switch. Nature 550:270-274 (2017). WB . PubMed: 28976960