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Cell Biology Cell Cycle Kinases/Phosphatases Other
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Axl overexpression 293T lysate (whole cell) (ab94145)

  • Datasheet
  • SDS
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SDS-PAGE - Axl overexpression 293T lysate (whole cell) (ab94145)
  • Western blot - Axl overexpression 293T lysate (whole cell) (ab94145)

Overview

  • Product name

    Axl overexpression 293T lysate (whole cell)
    See all Axl lysates
  • General notes

    ab94145 is a 293T cell transfected lysate in which Human Axl has been transiently over-expressed using a pCMV-Axl plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
  • Background

    Function: May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor. Tissue specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.

Applications

Our Abpromise guarantee covers the use of ab94145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • SDS-PAGE - Axl overexpression 293T lysate (whole cell) (ab94145)
    SDS-PAGE - Axl overexpression 293T lysate (whole cell) (ab94145)
    ab94145 at 15µg/lane on an SDS-PAGE gel.
  • Western blot - Axl overexpression 293T lysate (whole cell) (ab94145)
    Western blot - Axl overexpression 293T lysate (whole cell) (ab94145)
    All lanes : Anti-Axl antibody (ab54803) at 1/500 dilution

    Lane 1 : Axl overexpression 293T lysate (whole cell) (ab94145)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Protocols

  • Transfected Lysate Preparation Notes

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab94145? Please let us know so that we can cite the reference in this datasheet.

    ab94145 has not yet been referenced specifically in any publications.

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