- Datasheet
- Specific References
- Protocols
Overview
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Product nameAxl overexpression 293T lysate (whole cell)
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General notes
ab94145 is a 293T cell transfected lysate in which Human Axl has been transiently over-expressed using a pCMV-Axl plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applicationsSuitable for: WBmore details
Properties
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FormLiquid
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Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
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Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer -
Concentration information loading... -
PurityWhole Cell Lysate
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Research areas
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BackgroundFunction: May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor. Tissue specificity: Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily. Contains 2 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Applications
Our Abpromise guarantee covers the use of ab94145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. |
Images
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SDS-PAGE - Axl 293T Transfected Lysate - (positive control) (ab94145)ab94145 at 15µg/lane on an SDS-PAGE gel. -
Western blot - Axl 293T Transfected Lysate - (positive control) (ab94145)All lanes : Anti-Axl antibody (ab54803) at 1/500 dilution
Lane 1 :Axl overexpression 293T lysate (whole cell) (ab94145)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
Protocols
References
ab94145 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"