Recombinant Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61]
See all Dynein intermediate chain 1/DNAI1 primary antibodies -
Description
Rabbit monoclonal [EPR11244-61] to Dynein intermediate chain 1/DNAI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Dynein intermediate chain 1/DNAI1 aa 1-200. The exact sequence is proprietary.
Database link: Q9UI46 -
Positive control
- Human, rat and mouse testis lysates; Human testis and trachea tissues; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11244-61 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 79 kDa.
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ICC/IF |
1/50 - 1/100.
For unpurified, use 1/50 - 1/100. |
Notes |
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WB
1/2000. Predicted molecular weight: 79 kDa. |
ICC/IF
1/50 - 1/100. For unpurified, use 1/50 - 1/100. |
Target
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Function
Part of the dynein complex of respiratory cilia. -
Involvement in disease
Defects in DNAI1 are the cause of primary ciliary dyskinesia type 1 (CILD1) [MIM:244400]. CILD1 is an autosomal recessive disorder characterized by axonemal abnormalities of motile cilia. Respiratory infections leading to chronic inflammation and bronchiectasis are recurrent, due to defects in the respiratory cilia; reduced fertility is often observed in male patients due to abnormalities of sperm tails. Half of the patients exhibit situs inversus, due to dysfunction of monocilia at the embryonic node and randomization of left-right body asymmetry. Primary ciliary dyskinesia associated with situs inversus is referred to as Kartagener syndrome.
Defects in DNAI1 are the cause of Kartagener syndrome (KTGS) [MIM:244400]. KTGS is an autosomal recessive disorder characterized by the association of primary ciliary dyskinesia with situs inversus. Clinical features include recurrent respiratory infections, bronchiectasis, infertility, and lateral transposition of the viscera of the thorax and abdomen. The situs inversus is most often total, although it can be partial in some cases (isolated dextrocardia or isolated transposition of abdominal viscera). -
Sequence similarities
Belongs to the dynein intermediate chain family.
Contains 5 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton > cilium axoneme. - Information by UniProt
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Database links
- Entrez Gene: 27019 Human
- Entrez Gene: 68922 Mouse
- Entrez Gene: 500442 Rat
- Omim: 603772 Human
- SwissProt: Q9UI46 Human
- SwissProt: Q8C0M8 Mouse
- SwissProt: Q5XIL8 Rat
- Unigene: 112667 Human
see all -
Alternative names
- Axonemal dynein intermediate chain 1 antibody
- Axonemal dynein intermediate chain 2 antibody
- CILD 1 antibody
see all
Images
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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Human testis tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 79 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (purified) + Rat testis lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/5000 dilution (purified) + Mouse testis tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunofluorescence staining of F9 cells with purified ab171964 at a working dilution of 1 in 250, counter-stained with DAPI and mouse monoclonal anti-tubulin (ab7291, 1/500). The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom right hand and middle panels - for the negative control, purified ab171964 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500.
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All lanes : Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11244-61] (ab171964) at 1/1000 dilution (unpurified)
Lane 1 : Human testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lane 3 : Mouse testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 79 kDa -
Immunofluorescence analysis of Jurkat cells, labeling Dynein intermediate chain 1/DNAI1 using unpurified ab171964 at a 1/50 dilution (green) and DAPI nuclear staining (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab171964 has been referenced in 6 publications.
- Zubair M et al. A recurrent homozygous missense mutation in CCDC103 causes asthenoteratozoospermia due to disorganized dynein arms. Asian J Androl 24:255-259 (2022). PubMed: 35259782
- Guo T et al. Case Report: DNAAF4 Variants Cause Primary Ciliary Dyskinesia and Infertility in Two Han Chinese Families. Front Genet 13:934920 (2022). PubMed: 35903363
- Yang X et al. Time-dependent alterations in the rat nigrostriatal system after intrastriatal injection of fibrils formed by α-Syn and tau fragments. Front Aging Neurosci 14:1049418 (2022). PubMed: 36518823
- Li R et al. Fibroblast Growth Factor 13 Facilitates Peripheral Nerve Regeneration through Maintaining Microtubule Stability. Oxid Med Cell Longev 2021:5481228 (2021). PubMed: 34457114
- Jing Z et al. Rotavirus Viroplasm Biogenesis Involves Microtubule-Based Dynein Transport Mediated by an Interaction between NSP2 and Dynein Intermediate Chain. J Virol 95:e0124621 (2021). PubMed: 34379449
- Mali GR et al. ZMYND10 functions in a chaperone relay during axonemal dynein assembly. Elife 7:N/A (2018). PubMed: 29916806