Overview

  • Product name

    Anti-Axons of the Drosophila CNS antibody [BP102]
  • Description

    Mouse monoclonal [BP102] to Axons of the Drosophila CNS
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, IHC-FoFr, ICC, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Drosophila melanogaster
  • Immunogen

    Other Immunogen Type corresponding to Axons of the Drosophila CNS.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    BP102
  • Myeloma

    NS1
  • Isotype

    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab12455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
IHC-FoFr 1/1000.
ICC 1/1000.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Target

  • Alternative names

    • BP 102 Axons antibody
    • BP102 Axons antibody
    • Drosophila CNS axons antibody

Images

  • ab12455 used in immunofluorescence at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

    Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining.

  • ab12455 used in immunohistochemistry at 1/1000 on Drosophila embryos. The protocol used is available in the Neuroscience Abwire in the protocols section.

    Briefly, embryos were fixed with PBS, formaldehyde and n-heptane. Embryos were then dechorionated with 100% bleach and rehydrated with PBT (PBS with 0.3% Tritone added) before antibody staining. Visualisation was with alkaline phosphatase.

  • ab12455 staining axons in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a solution that contains 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/1000) in a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS at 40C for 12 hours. Cyt3®-conjugated donkey polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.

    See Abreview

References

This product has been referenced in:

See all 17 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Blockace/0.05% Triton X-100/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 08 2010

Answer

Thank you for your enquiry. Unfortunately, we are unable to obtain the amino acid sequence for this product as the information is proprietary. I am afraid that all the information we have is already stated on the datasheet. I am sorry I can't be of more assistance in this occasion but should you require anything else, please do not hesitate to contact us again.

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