Key features and details
- Rabbit polyclonal to AZI1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AZI1 antibody
See all AZI1 primary antibodies
DescriptionRabbit polyclonal to AZI1
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Immunogen corresponds to a region between residues 1033 and 1083 of Human AZI1 (EAW89632.1)
- Hela whole cell lysate and 293T cell lysate
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 122 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionMay play a role in spermatogenesis.
Sequence similaritiesContains 1 IQ domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- 5 azacytidine induced 1 antibody
- 5-azacytidine-induced protein 1 antibody
- AZ1 antibody
All lanes : Anti-AZI1 antibody (ab99315) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 10 seconds
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99315 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
ab99315 at 1 µg/ml detecting AZI1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of AZI1
Lane 2: IP with an another antibody which recognizes an upstream epitope of AZI1
Lane 3: ab99315 at 3µg/mg of lysate
Lane 4: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds
ab99315 has not yet been referenced specifically in any publications.