Product nameAnti-AZI1 antibody
See all AZI1 primary antibodies
DescriptionRabbit polyclonal to AZI1
Tested applicationsSuitable for: WB, IP, ICC/IF, ICCmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Immunogen corresponds to a region between residues 1033 and 1083 of Human AZI1 (EAW89632.1)
- Hela whole cell lysate and 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 122 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ICC/IF||1/100 - 1/500.|
|ICC||1/100 - 1/500.
Formaldehyde fixation with Triton-X 100 permeabilization is recommended.
FunctionMay play a role in spermatogenesis.
Sequence similaritiesContains 1 IQ domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- 5 azacytidine induced 1 antibody
- 5-azacytidine-induced protein 1 antibody
- AZ1 antibody
All lanes : Anti-AZI1 antibody (ab99315) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 10 seconds
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99315 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
ab99315 at 1 µg/ml detecting AZI1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of AZI1
Lane 2: IP with an another antibody which recognizes an upstream epitope of AZI1
Lane 3: ab99315 at 3µg/mg of lysate
Lane 4: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds
ab99315 has not yet been referenced specifically in any publications.