Anti-AZI1 antibody (ab99379)
Key features and details
- Rabbit polyclonal to AZI1
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AZI1 antibody
See all AZI1 primary antibodies -
Description
Rabbit polyclonal to AZI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human AZI1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. EAW89632.1
Database link: Q9UPN4 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
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IP | (1) |
Use at 2-5 µg/mg of lysate.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 122 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ICC/IF
1/100 - 1/500. |
Target
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Function
May play a role in spermatogenesis. -
Sequence similarities
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 22994 Human
- Omim: 613479 Human
- SwissProt: Q9UPN4 Human
- Unigene: 514578 Human
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Alternative names
- 5 azacytidine induced 1 antibody
- 5-azacytidine-induced protein 1 antibody
- AZ1 antibody
see all
Images
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All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 122 kDa
Exposure time: 30 seconds -
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
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Detection of AZI1 by Western Blot of Immunprecipitate.
ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab99379 has been referenced in 7 publications.
- Ando K et al. CEP131 Abrogates CHK1 Inhibitor-Induced Replication Defects and Is Associated with Unfavorable Outcome in Neuroblastoma. J Oncol 2020:2752417 (2020). PubMed: 33014050
- Shang Z et al. USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis. J Biol Chem 294:9844-9857 (2019). PubMed: 31073027
- Holdgaard SG et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
- Li X et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB, IP . PubMed: 28361952
- Airik R et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS One 11:e0156081 (2016). PubMed: 27224062
- Staples CJ et al. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability. J Cell Sci 127:2910-9 (2014). WB . PubMed: 24816561
- Staples CJ et al. The centriolar satellite protein Cep131 is important for genome stability. J Cell Sci 125:4770-9 (2012). PubMed: 22797915