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    azi1-antibody-ab99379.pdf

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Tags & Cell Markers Subcellular Markers Organelles Centrosome
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Anti-AZI1 antibody (ab99379)

  • Datasheet
Reviews (4) Submit a question References (6)

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Abpromise

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Find out more.

Western blot - Anti-AZI1 antibody (ab99379)
  • Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)
  • Immunoprecipitation - Anti-AZI1 antibody (ab99379)

Key features and details

  • Rabbit polyclonal to AZI1
  • Suitable for: WB, IP, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-AZI1 antibody
    See all AZI1 primary antibodies
  • Description

    Rabbit polyclonal to AZI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human AZI1 (EAW89632.1).

  • Positive control

    • HeLa and 293T whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab99379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF 1/100 - 1/500.

Target

  • Function

    May play a role in spermatogenesis.
  • Sequence similarities

    Contains 1 IQ domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome.
  • Target information above from: UniProt accession Q9UPN4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 22994 Human
    • Omim: 613479 Human
    • SwissProt: Q9UPN4 Human
    • Unigene: 514578 Human
    • Alternative names

      • 5 azacytidine induced 1 antibody
      • 5-azacytidine-induced protein 1 antibody
      • AZ1 antibody
      • Azi antibody
      • Azi1 antibody
      • AZI1_HUMAN antibody
      • Centrosomal protein 131 kDa antibody
      • Centrosomal protein of 131 kDa antibody
      • Cep131 antibody
      • KIAA1118 antibody
      • OTTMUSP00000004498 antibody
      • Pre-acrosome localization protein 1 antibody
      • RP23 37J21.1 antibody
      see all

    Images

    • Western blot - Anti-AZI1 antibody (ab99379)
      Western blot - Anti-AZI1 antibody (ab99379)
      All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 122 kDa


      Exposure time: 30 seconds
    • Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)
      Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)
      Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
    • Immunoprecipitation - Anti-AZI1 antibody (ab99379)
      Immunoprecipitation - Anti-AZI1 antibody (ab99379)
      Detection of AZI1 by Western Blot of Immunprecipitate.
      ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    Protocols

    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (6)

    Publishing research using ab99379? Please let us know so that we can cite the reference in this datasheet.

    ab99379 has been referenced in 6 publications.

    • Shang Z  et al. USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis. J Biol Chem 294:9844-9857 (2019). PubMed: 31073027
    • Holdgaard SG  et al. Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites. Nat Commun 10:4176 (2019). PubMed: 31519908
    • Li X  et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB, IP . PubMed: 28361952
    • Airik R  et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS One 11:e0156081 (2016). PubMed: 27224062
    • Staples CJ  et al. Ccdc13 is a novel human centriolar satellite protein required for ciliogenesis and genome stability. J Cell Sci 127:2910-9 (2014). WB . PubMed: 24816561
    • Staples CJ  et al. The centriolar satellite protein Cep131 is important for genome stability. J Cell Sci 125:4770-9 (2012). PubMed: 22797915

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunoprecipitation abreview for Anti-AZI1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein G
    Specification
    U2OS cells
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 15 2017

    Western blot abreview for Anti-AZI1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Different human tissue culture cell lines)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage)
    Loading amount
    40 µg
    Specification
    Different human tissue culture cell lines
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Western blot abreview for Anti-AZI1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (MEFs and mouse brain lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage)
    Loading amount
    40 µg
    Specification
    MEFs and mouse brain lysate
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Western blot abreview for Anti-AZI1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 10 2012

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