Overview

  • Product name
    Anti-AZI1 antibody
    See all AZI1 primary antibodies
  • Description
    Rabbit polyclonal to AZI1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human AZI1 (EAW89632.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 122 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC 1/100 - 1/500.

Formaldehyde fixation with Triton-X 100 permeabilization is recommended.
ICC/IF 1/100 - 1/500.

Target

  • Function
    May play a role in spermatogenesis.
  • Sequence similarities
    Contains 1 IQ domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
    • Alternative names
      • 5 azacytidine induced 1 antibody
      • 5-azacytidine-induced protein 1 antibody
      • AZ1 antibody
      • Azi antibody
      • Azi1 antibody
      • AZI1_HUMAN antibody
      • Centrosomal protein 131 kDa antibody
      • Centrosomal protein of 131 kDa antibody
      • Cep131 antibody
      • KIAA1118 antibody
      • OTTMUSP00000004498 antibody
      • Pre-acrosome localization protein 1 antibody
      • RP23 37J21.1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)
      Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)
      Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
    • Western blot - Anti-AZI1 antibody (ab99379)
      Western blot - Anti-AZI1 antibody (ab99379)
      All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 122 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-AZI1 antibody (ab99379)
      Immunoprecipitation - Anti-AZI1 antibody (ab99379)
      Detection of AZI1 by Western Blot of Immunprecipitate.
      ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    This product has been referenced in:
    • Li X  et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB, IP . Read more (PubMed: 28361952) »
    • Airik R  et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS One 11:e0156081 (2016). Read more (PubMed: 27224062) »

    See all 3 Publications for this product

    Publishing research using ab99379? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    4 reviews or Q&A

    10
    Votes: Highest First

    Immunoprecipitation abreview for Anti-AZI1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein G
    Specification
    U2OS cells
    Read More
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    Abcam user community

    Verified customer

    Submitted Mar 15 2017

    Western blot abreview for Anti-AZI1 antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Different human tissue culture cell lines)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage)
    Loading amount
    40 µg
    Specification
    Different human tissue culture cell lines
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Western blot abreview for Anti-AZI1 antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (MEFs and mouse brain lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage)
    Loading amount
    40 µg
    Specification
    MEFs and mouse brain lysate
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted Jan 18 2017

    Western blot abreview for Anti-AZI1 antibody

     Average
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS cells)
    Loading amount
    50 µg
    Specification
    U2OS cells
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Oct 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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