Anti-AZI1 antibody (ab99379)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-AZI1 antibody
See all AZI1 primary antibodies -
Description
Rabbit polyclonal to AZI1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human AZI1 (EAW89632.1).
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab99379 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 122 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| ICC | 1/100 - 1/500. Formaldehyde fixation with Triton-X 100 permeabilization is recommended. |
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| ICC/IF | 1/100 - 1/500. |
Target
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Function
May play a role in spermatogenesis. -
Sequence similarities
Contains 1 IQ domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. - Information by UniProt
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Database links
- Entrez Gene: 22994 Human
- Omim: 613479 Human
- SwissProt: Q9UPN4 Human
- Unigene: 514578 Human
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Alternative names
- 5 azacytidine induced 1 antibody
- 5-azacytidine-induced protein 1 antibody
- AZ1 antibody
see all
Images
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Western blot - Anti-AZI1 antibody (ab99379)All lanes : Anti-AZI1 antibody (ab99379) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 122 kDa
Exposure time: 30 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-AZI1 antibody (ab99379)Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling AZI1 with ab99379 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody. -
Immunoprecipitation - Anti-AZI1 antibody (ab99379)Detection of AZI1 by Western Blot of Immunprecipitate.
ab99379 at 1µg/ml staining AZI1 in HeLa whole cell lysate immunoprecipitated using ab99379 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Shang Z et al. USP9X-mediated deubiquitination of B-cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis. J Biol Chem 294:9844-9857 (2019). Read more (PubMed: 31073027) »
- Li X et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). WB, IP . Read more (PubMed: 28361952) »
