Overview

  • Product name

    Anti-AZI2 antibody [EPR14698] - C-terminal
    See all AZI2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14698] to AZI2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human AZI2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9H6S1

  • Positive control

    • HeLa, 293, NIH 3T3 and Rat kidney lysate; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFN-beta promoter activation via TICAM1.
  • Tissue specificity

    Widely expressed. Abundant expression seen in the pancreas and testis.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 azacytidine induced protein 2 antibody
    • 5-azacytidine-induced protein 2 antibody
    • AA410145 antibody
    • AZ2 antibody
    • AZI2 antibody
    • AZI2_HUMAN antibody
    • MGC112644 antibody
    • Nak associated protein 1 antibody
    • Nak-associated protein 1 antibody
    • NAP1 antibody
    • NF kappa B activating kinase associated protein 1 antibody
    • NF-kappa-B-activating kinase-associated protein 1 antibody
    • TILP antibody
    • TILP(S) antibody
    see all

Images

  • Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/5000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling AZI2 with ab192253 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) ab150078 was used as the secondary at 1/500 dilution.  Couunterstain DAPI. 

    Negative controls: anti-AZI2 at 1/100 dilution, Secondary ab Goat anti mouse IgG (Alexa Fluor®488 at 1/200 dilution.

  • Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/1000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

  • All lanes : Anti-AZI2 antibody [EPR14698] - C-terminal (ab192253) at 1/1000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 45 kDa

References

ab192253 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192253.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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