Anti-Azurocidin antibody (ab167336)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Azurocidin antibody
See all Azurocidin primary antibodies -
DescriptionMouse polyclonal to Azurocidin
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Full length protein, corresponding to amino acids 1-251 of Human Azurocidin (NP_001691.1).
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Positive control
- Human spleen lysate; Azurocidin transfected 293T cell lysate; Human lymph node tissue; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab167336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionThis is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
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Sequence similaritiesBelongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. -
Cellular localizationCytoplasmic granule. Cytoplasmic granules of neutrophils.
- Information by UniProt
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Database links
- Entrez Gene: 566 Human
- Omim: 162815 Human
- SwissProt: P20160 Human
- Unigene: 72885 Human
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Alternative names
- Azu 1 antibody
- AZU1 antibody
- Azurocidin antibody
see all
Images
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Western blot - Anti-Azurocidin antibody (ab167336)Anti-Azurocidin antibody (ab167336) at 1 µg/ml + Human spleen lysate at 50 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa -
Western blot - Anti-Azurocidin antibody (ab167336)All lanes : Anti-Azurocidin antibody (ab167336) at 1 µg/ml
Lane 1 : Azurocidin transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution
Predicted band size: 26 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Azurocidin antibody (ab167336)Immunofluorescent analysis of Azurocidin expression in HeLa cells using ab167336 at 10 µg/ml dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody (ab167336)Immunohistochemical analysis of Azurocidin expression in formalin-fixed paraffin-embedded Human lymph node tissue using ab167336 at 3 µg/ml dilution
Protocols
Datasheets and documents
References
This product has been referenced in:
- Mayer P et al. Changes in the microarchitecture of the pancreatic cancer stroma are linked to neutrophil-dependent reprogramming of stellate cells and reflected by diffusion-weighted magnetic resonance imaging. Theranostics 8:13-30 (2018). Read more (PubMed: 29290790) »
