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    azurocidin-antibody-ab167336.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Azurocidin antibody (ab167336)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-Azurocidin antibody (ab167336)
  • Western blot - Anti-Azurocidin antibody (ab167336)
  • Immunocytochemistry/ Immunofluorescence - Anti-Azurocidin antibody (ab167336)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody (ab167336)

Key features and details

  • Mouse polyclonal to Azurocidin
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-Azurocidin antibody
    See all Azurocidin primary antibodies
  • Description

    Mouse polyclonal to Azurocidin
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Full length protein, corresponding to amino acids 1-251 of Human Azurocidin (NP_001691.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human spleen lysate; Azurocidin transfected 293T cell lysate; Human lymph node tissue; HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.4
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Microbiology
    • Protein
    • Human Protein
    • Defensin
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines
    • Microbiology
    • Antimicrobial
    • Antibiotic

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG - Isotype Control (ab37355)
  • Recombinant Protein

    • Recombinant Human Azurocidin protein (ab179973)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
IHC-P
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of neutrophils.
  • Target information above from: UniProt accession P20160 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 566 Human
    • Omim: 162815 Human
    • SwissProt: P20160 Human
    • Unigene: 72885 Human
    • Alternative names

      • Azu 1 antibody
      • AZU1 antibody
      • Azurocidin antibody
      • CAP37 antibody
      • CAP7_HUMAN antibody
      • Cationic antimicrobial protein 37 antibody
      • Cationic antimicrobial protein CAP37 antibody
      • HBP antibody
      • Heparin binding protein antibody
      • Heparin-binding protein antibody
      • HUMAZUR antibody
      • Neutrophil azurocidin antibody
      see all

    Images

    • Western blot - Anti-Azurocidin antibody (ab167336)
      Western blot - Anti-Azurocidin antibody (ab167336)
      Anti-Azurocidin antibody (ab167336) at 1 µg/ml + Human spleen lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG HRP at 1/2500 dilution

      Predicted band size: 26 kDa

    • Western blot - Anti-Azurocidin antibody (ab167336)
      Western blot - Anti-Azurocidin antibody (ab167336)
      All lanes : Anti-Azurocidin antibody (ab167336) at 1 µg/ml

      Lane 1 : Azurocidin transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution

      Predicted band size: 26 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Azurocidin antibody (ab167336)
      Immunocytochemistry/ Immunofluorescence - Anti-Azurocidin antibody (ab167336)
      Immunofluorescent analysis of Azurocidin expression in HeLa cells using ab167336 at 10 µg/ml dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody (ab167336)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Azurocidin antibody (ab167336)
      Immunohistochemical analysis of Azurocidin expression in formalin-fixed paraffin-embedded Human lymph node tissue using ab167336 at 3 µg/ml dilution

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab167336? Please let us know so that we can cite the reference in this datasheet.

    ab167336 has been referenced in 1 publication.

    • Mayer P  et al. Changes in the microarchitecture of the pancreatic cancer stroma are linked to neutrophil-dependent reprogramming of stellate cells and reflected by diffusion-weighted magnetic resonance imaging. Theranostics 8:13-30 (2018). PubMed: 29290790

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