Overview

  • Product name
  • Description
    Mouse polyclonal to Azurocidin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-251 of Human Azurocidin (NP_001691.1).

  • Positive control
    • Human spleen lysate; Azurocidin transfected 293T cell lysate; Human lymph node tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab167336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • Sequence similarities
    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Cytoplasmic granule. Cytoplasmic granules of neutrophils.
  • Information by UniProt
  • Database links
  • Alternative names
    • Azu 1 antibody
    • AZU1 antibody
    • Azurocidin antibody
    • CAP37 antibody
    • CAP7_HUMAN antibody
    • Cationic antimicrobial protein 37 antibody
    • Cationic antimicrobial protein CAP37 antibody
    • HBP antibody
    • Heparin binding protein antibody
    • Heparin-binding protein antibody
    • HUMAZUR antibody
    • Neutrophil azurocidin antibody
    see all

Images

  • Anti-Azurocidin antibody (ab167336) at 1 µg/ml + Human spleen lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 26 kDa

  • All lanes : Anti-Azurocidin antibody (ab167336) at 1 µg/ml

    Lane 1 : Azurocidin transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 26 kDa

  • Immunofluorescent analysis of Azurocidin expression in HeLa cells using ab167336 at 10 µg/ml dilution.
  • Immunohistochemical analysis of Azurocidin expression in formalin-fixed paraffin-embedded Human lymph node tissue using ab167336 at 3 µg/ml dilution

References

This product has been referenced in:
  • Mayer P  et al. Changes in the microarchitecture of the pancreatic cancer stroma are linked to neutrophil-dependent reprogramming of stellate cells and reflected by diffusion-weighted magnetic resonance imaging. Theranostics 8:13-30 (2018). Read more (PubMed: 29290790) »
See 1 Publication for this product

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