The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FACS: Use Neat.
Use 10ul of neat antibody to label 106 cells or cells or 100ul whole blood.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
B cells are lymphocytes that are produced in the bone marrow and require bone marrow stromal cells and their cytokines for maturation. During its development, each B cell becomes genetically programmed through a series of gene splicing reactions to produce an antibody molecule with a unique specificity.