Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab12296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/2500. Detects a band of approximately ~90 kDa (predicted molecular weight: 79 kDa).

    ab12296 was tested in ICC/IF on HeLa cells (1/100, 1/200, 1/500) and gave high background staining. It appears the antibody may not work in this application.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
    • Sequence similarities
      Contains 3 HTH myb-type DNA-binding domains.
    • Post-translational
      modifications
      Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • B-Myb antibody
      • BMyB antibody
      • MGC15600 antibody
      • MYB L2 antibody
      • Myb related protein B antibody
      • Myb-like protein 2 antibody
      • Myb-related protein B antibody
      • MybB antibody
      • MYBB_HUMAN antibody
      • MYBL 2 antibody
      • Mybl2 antibody
      • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
      • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
      • v myb myeloblastosis viral oncogene homolog like 2 antibody
      see all

    Images

    • A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.

      B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.

      A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.

      ab12296 dilution: 200 µg/ml.
      Exposure was for 10 minutes.

       

       

      A: 293 HEK cells were transfected with negative control cDNA (pcDNA3 vector) or human B-Myb cDNA.
      B: negative control cDNA (pcDNA3 vector) or human B-Myb cDNA were in vitro translated in rabbit reticulocyte lysate.
      A band corresponding to B-Myb was detected (arrow) in all lanes except negative control lanes.
      ab12296 dilution: 200 µg/ml. Exposure was for 10 minutes.
    • Anti-B MyB antibody (ab12296) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing B-Myb at 5 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 79 kDa
      Observed band size: 98 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 3 minutes

    References

    This product has been referenced in:
    • Porta S  et al. Drosha inclusions are new components of dipeptide-repeat protein aggregates in FTLD-TDP and ALS C9orf72 expansion cases. J Neuropathol Exp Neurol 74:380-7 (2015). Read more (PubMed: 25756586) »
    • Heinrichs S  et al. MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy. Elife 2:e00825 (2013). WB ; Human . Read more (PubMed: 23878725) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela cells)
    Specification
    Hela cells
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Fish scale gelatin in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 02 2010

    Question
    Answer

    Thank you for your enquiry and patience. I'm still trying to obtain information about whether the antibody was tested with untransfected 293 HEK cells (with regard to the image on the online datasheet), and will let you know if I am able to obtain this information. From the information that I could gather, ab12296 does appear to detect endogenous B-Myb in various cell lines (HeLa, 293, U20S, MOLT-4, and COS-7). B-Myb is seen at approximately 90 kDa but there are also some additional bands seen (at approximately 100 and 59 kDa). I hope this is helpful, if you have any additional questions please contact us again.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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