Product nameAnti-B MyB antibody
See all B MyB primary antibodies
DescriptionGoat polyclonal to B MyB
Tested applicationsSuitable for: WB, IHC-Fr, ELISAmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Cow, Human
- Purchase matching WB positive control:Recombinant Human B MyB protein
- Rat stomach lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab53511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 79 kDa).|
|IHC-Fr||1/300. PubMed: 24886974|
|ELISA||Use at an assay dependent concentration.
Peptide ELISA: antibody detection limit dilution 1:32,000.
FunctionTranscription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
modificationsPhosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
- Information by UniProt
- B-Myb antibody
- BMyB antibody
- MGC15600 antibody
Anti-B MyB antibody (ab53511) at 0.1 µg/ml + Rat stomach lysate (35µg protein in RIPA buffer)
Predicted band size: 79 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.