Key features and details
- Rabbit polyclonal to B MyB (phospho S577 + S581)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-B MyB (phospho S577 + S581) antibody
See all B MyB primary antibodies
DescriptionRabbit polyclonal to B MyB (phospho S577 + S581)
SpecificityDetects endogenous levels of B MyB only when phosphorylated at serine 577/581.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic phosphopeptide derived from human B MyB around the phosphorylation site of serine 577/581 (R-R-SP-P-I).
- Purchase matching WB positive control:Recombinant Human B MyB protein
- Extracts from K562 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab61000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).|
FunctionTranscription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
Sequence similaritiesContains 3 HTH myb-type DNA-binding domains.
modificationsPhosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
- Information by UniProt
- B-Myb antibody
- BMyB antibody
- MGC15600 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab61000 has been referenced in 1 publication.
- Werwein E et al. B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress. Cell Death Dis 4:e511 (2013). ICC/IF ; Mouse, Human . PubMed: 23449447