Recombinant Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009)


  • Product name
    Anti-B MyB (phospho T487) antibody [EPR2204Y]
    See all B MyB primary antibodies
  • Description
    Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487)
  • Host species
  • Specificity
    ab76009 detects B MyB phosphorlyated on threonine 487.
  • Tested applications
    Suitable for: ChIP, ICC/IF, Flow Cyt, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding threonine 487 of human B MyB.

  • Positive control
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab76009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 22391450
ICC/IF 1/100 - 1/250.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/1000. Predicted molecular weight: 79 kDa.
IP 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.


  • Function
    Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similarities
    Contains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all


  • ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.
  • All lanes : Anti-B MyB (phospho T487) antibody [EPR2204Y] (ab76009) at 1/1000 dilution

    Lane 1 : Raji cell lysate, untreated
    Lane 2 : Raji cell lysate treated with TPA + IGF

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 79 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.
  • Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


This product has been referenced in:
  • Werwein E  et al. Activation of the oncogenic transcription factor B-Myb via multisite phosphorylation and prolyl cis/trans isomerization. Nucleic Acids Res 47:103-121 (2019). Read more (PubMed: 30321399) »
  • Qiao A  et al. The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol 216:723-741 (2017). Read more (PubMed: 28183717) »
See all 6 Publications for this product

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Dog Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1

Mr. Emmanuel Bouchaert

Verified customer

Submitted Jun 30 2017

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