Recombinant
RabMAb

Recombinant Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free (ab232521)

Overview

  • Product name

    Anti-B MyB (phospho T487) antibody [EPR2204Y] - BSA and Azide free
    See all B MyB primary antibodies
  • Description

    Rabbit monoclonal [EPR2204Y] to B MyB (phospho T487) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab232521 detects B MyB phosphorlyated on threonine 487.
  • Tested applications

    Suitable for: ChIP, WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human B MyB aa 450-550 (phospho T487). The exact sequence is proprietary.
    Database link: P10244

  • Positive control

    • IHC-P: Human urinary bladder carcinoma tissue.
  • General notes

    Ab232521 is the carrier-free version of ab76009. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232521 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.

Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.
  • Sequence similarities

    Contains 3 HTH myb-type DNA-binding domains.
  • Post-translational
    modifications

    Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B-Myb antibody
    • BMyB antibody
    • MGC15600 antibody
    • MYB L2 antibody
    • Myb related protein B antibody
    • Myb-like protein 2 antibody
    • Myb-related protein B antibody
    • MybB antibody
    • MYBB_HUMAN antibody
    • MYBL 2 antibody
    • Mybl2 antibody
    • v myb avian myeloblastosis viral oncogene homolog like 2 antibody
    • v myb myeloblastosis viral oncogene homolog (avian) like 2 antibody
    • v myb myeloblastosis viral oncogene homolog like 2 antibody
    see all

Images

  • ab76009, at 1/100 dilution, staining B MyB in human urinary bladder carcinoma by immunohistochemistry using paraffin-embedded tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76009).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab76009, at 1/100 dilution, staining B MyB in HeLa cells by immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76009).

  • Overlay histogram showing HeLa cells stained with ab76009 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76009, 1/50 dilution) for 30 min at 22�C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22�C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76009).

References

ab232521 has not yet been referenced specifically in any publications.

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