Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP643Y] to b-Myc - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Product nameAnti-b-Myc antibody [EP643Y] - BSA and Azide free
See all b-Myc primary antibodies
DescriptionRabbit monoclonal [EP643Y] to b-Myc - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat
Synthetic peptide within Rat b-Myc aa 50-150. The exact sequence is proprietary.
Ab247291 is the carrier-free version of ab40798. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247291 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab247291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 18.3 kDa).|
Relevanceb-Myc is a member of the myc family of transcriptional regulators including c-myc, N-myc, L-myc and S-myc. Even though the biological role of b-myc has not yet been determined, b-myc has been show to inhibit c-myc function. Mostly expressed in the brain, protein expression in adrenal gland, pituitary gland, mammary gland, ovary, and uterus suggest that b-myc gene expression is associated with hormonally regulated tissues.
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All lanes : Anti-b-Myc antibody [EP643Y] (ab40798) at 1/500 dilution
Lane 1 : Rat Brain
Lane 2 : PC12
Predicted band size: 18.3 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
This data was developed using ab40798, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab247291 has not yet been referenced specifically in any publications.