Recombinant Anti-b-Myc antibody [EP643Y] - BSA and Azide free (ab247291)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP643Y] to b-Myc - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat
Related conjugates and formulations
Overview
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Product name
Anti-b-Myc antibody [EP643Y] - BSA and Azide free
See all b-Myc primary antibodies -
Description
Rabbit monoclonal [EP643Y] to b-Myc - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247291 is the carrier-free version of ab40798.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP643Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 18.3 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 20 kDa (predicted molecular weight: 18.3 kDa). |
Target
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Relevance
b-Myc is a member of the myc family of transcriptional regulators including c-myc, N-myc, L-myc and S-myc. Even though the biological role of b-myc has not yet been determined, b-myc has been show to inhibit c-myc function. Mostly expressed in the brain, protein expression in adrenal gland, pituitary gland, mammary gland, ovary, and uterus suggest that b-myc gene expression is associated with hormonally regulated tissues. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 107771 Mouse
- Entrez Gene: 311807 Rat
- SwissProt: Q6P8Z1 Mouse
- SwissProt: P15063 Rat
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Alternative names
- 2900002K07Rik antibody
- AW060705 antibody
- Brain expressed myelocytomatosis oncogene antibody
- Mycb antibody
Images
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All lanes : Anti-b-Myc antibody [EP643Y] (ab40798) at 1/500 dilution
Lane 1 : Rat Brain
Lane 2 : PC12
Predicted band size: 18.3 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?This data was developed using ab40798, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247291 has not yet been referenced specifically in any publications.