Product nameAnti-B Phycoerythrin antibody
See all B Phycoerythrin primary antibodies
DescriptionRabbit polyclonal to B Phycoerythrin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Species independent
Full length native protein (purified) corresponding to B Phycoerythrin. Immunogen: Phycoerythrin alpha chain [Porphyridium creuentum]
Database link: P11392
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181711 is purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the stated buffer. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].
Our Abpromise guarantee covers the use of ab181711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/1000 - 1/5000.|
RelevanceB-Phycoerythrin (B-PE), a fluorescent protein from phycobiliprotein family, is isolated from cyanobacteria and eukaryotic algae. Its primary absorption peak is at 545 nm with a secondary peak at 563 nm.
- B-phycoerythrin alpha chain antibody
- B-phycoerythrin beta chain antibody
- cpeA antibody
- cpeB antibody
All lanes : Anti-B Phycoerythrin antibody (ab181711) at 1/1000 dilution
All lanes : Phycoerythrin (reduced by adding 4% BME and boiling for 5 minute)
Lysates/proteins at 1 µg per lane.
All lanes : Donkey anti-rabbit antibody at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
ab181711 has not yet been referenced specifically in any publications.