Product nameB-Phycoerythrin Conjugation Kit - Lightning-Link®
B-Phycoerythrin Conjugation Kit ab102871 uses a simple and quick process for B-PE labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.
To conjugate an antibody to B-Phycoerythrin using this kit:
- add modifier to antibody and incubate for 3 hrs
- add quencher and incubate for 30 mins
The B-PE conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to B-Phycoerythrin.
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link® B-PE Labeling Kit. 716-0015 is the same as the 600 µg size. 716-0010 is the same as the 3 x 60 ug size. 716-0005 is the same as the 60 µg size.
Amount and volume of antibody for conjugation to B-Phycoerythrin.
Kit size Recommended
amount of antibody
3 x 60 µg 3 x 60 µg 3 x 60 µL 60 µg 1 x 60 µg 1 x 60 µL 600 µg 1 x 600 µg 1 x 600 µL
1 Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 1 mg/ml or < 0.5 mg/ml should be diluted /concentrated.
The selling size of this product has been changed – it is now based on the amount of antibody that can be conjugated with the kit, not the amount of PE mix provided. The quantity of reagents provided have not changed or the amount of antibody that can be conjugated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris1 0.1% BSA 50% glycerol 0.1% sodium azide PBS Potassium phosphate Sodium chloride HEPES Sucrose Sodium citrate EDTA Trehalose
1 Tris buffered saline is almost always ≤ 50 mM / 0.6%
Incompatible buffer constituents
Thiomerosal Proclin Glycine Arginine Glutathione DTT
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Tested applicationsSuitable for: Conjugationmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 60 µg 600 µg 3 x 60 µg ab274139 - BPE mix 1 x 60µg 1 x 600µg 3 x 60µg ab274106 - Modifier reagent 1 x 200µl 1 x 200µl 1 x 200µl ab274296 - Quencher reagent 1 x 200µl 1 x 200µl 1 x 200µl
- B PE
Our Abpromise guarantee covers the use of ab102871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent dilution.|
ab102871 has been referenced in 1 publication.
- Jung M et al. Supplementation of dietary germanium biotite enhances induction of the immune responses by foot-and-mouth disease vaccine in cattle. BMC Vet Res 10:179 (2014). PubMed: 25255918