Overview

  • Product name

  • Description

    Rabbit polyclonal to B19
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human B19 aa 650-714 (C terminal).
    Database link: P57058

  • Positive control

    • A431 and HeLa whole cell lysate (ab150035); A549 cells; Human OVCA xenograft tissue.
  • General notes

    Previously labelled as HUNK.

Properties

Applications

Our Abpromise guarantee covers the use of ab137492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH 8
ICC/IF 1/100 - 1/1000.

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • B19 antibody
    • Bstk1 antibody
    • Hormonally up-regulated neu tumor-associated kinase antibody
    • Hormonally upregulated neu associated kinase antibody
    • Hormonally upregulated neu tumor associated kinase antibody
    • HUNK antibody
    • HUNK_HUMAN antibody
    • MAKV antibody
    • Serine/threonine-protein kinase MAK-V antibody
    see all

Images

  • Anti-B19 antibody (ab137492) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labelling B19 with ab137492 at 1/200 dilution. The lower image is costained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue labelling B19 with ab137492 at 1/100 dilution.

References

ab137492 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137492.
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