Key features and details
- Rabbit polyclonal to B19
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-B19 antibody
See all B19 primary antibodies
DescriptionRabbit polyclonal to B19
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human B19 aa 650-714 (C terminal).
Database link: P57058
- A431 and HeLa whole cell lysate (ab150035); A549 cells; Human OVCA xenograft tissue.
Previously labelled as HUNK.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 80 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer pH 8|
|ICC/IF||1/100 - 1/1000.|
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- B19 antibody
- Bstk1 antibody
- Hormonally up-regulated neu tumor-associated kinase antibody
Anti-B19 antibody (ab137492) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed A549 cells labelling B19 with ab137492 at 1/200 dilution. The lower image is costained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue labelling B19 with ab137492 at 1/100 dilution.
ab137492 has not yet been referenced specifically in any publications.