Anti-B1R antibody (ab75148)
Key features and details
- Rabbit polyclonal to B1R
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B1R antibody
See all B1R primary antibodies -
Description
Rabbit polyclonal to B1R -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human B1R (internal sequence).
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Positive control
- Extracts from K562 cells. LOVO cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 623 Human
- Omim: 600337 Human
- SwissProt: P46663 Human
- Unigene: 525572 Human
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Alternative names
- B1 bradykinin receptor antibody
- B1BKR antibody
- B1R antibody
see all
Images
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All lanes : Anti-B1R antibody (ab75148) at 1/500 dilution
Lane 1 : Extracts from K562 cells
Lane 2 : Extracts from K562 cells with immunising peptide at 5 µg
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 48 kDa, 50 kDa, 82 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of B1R in LOVO cells using ab75148, at 1/500 dilution, with (right panel) or without (left panel) preincubation with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab75148 has been referenced in 2 publications.
- Meng X et al. Involvement of bradykinin and bradykinin B1 receptor in patients with endometriosis. Exp Ther Med 22:1240 (2021). PubMed: 34539836
- Ghebrehiwet B et al. Soluble gC1qR is an autocrine signal that induces B1R expression on endothelial cells. J Immunol 192:377-84 (2014). WB . PubMed: 24319267